激素对小麦幼胚胚性无性系高频率诱导的影响

Effect of exogenous hormones on high frequency induction of embryogenic somaclone derived from immature embryos of wheat ( Triticum aestivum L.)

研究了 3种外源激素对 3个小麦品种 (系 )幼胚体细胞胚性无性系高频率形成的影响。结果表明 :2 ,4- D能显著促进小麦幼胚体细胞胚性无性系的形成 ,并存在着基因型间的差异 ,在小麦体细胞无性系形成中起主要作用。其最佳适用质量浓度为 2 .0～ 3.0 m g/ L ,2 ,4- D的这种作用能被培养基中加入的细胞分裂素所加强 ,这种作用在不同的细胞分裂素间是有差异的 ,在质量浓度 0 .1～ 2 .0 m g· L- 1 内 ,随 KT浓度的升高 ,6 - BA浓度的降低 。

Effect of three exogenous hormones on high frequency induction of three varieties of wheat ( Triticum aestivum L.) immature embryos is studied.The following results are acquired.Concentration of 2,4 D in media significantly affects the induction of embryogenic calli derived from immature embryos of wheat,with different frequency between the three genotypes.The most appropriate concentration of 2,4 D is at 2.0 ～3.0 mg/L.This capability of 2,4 D can be enforced by the addition of KT or 6 BA in medium.But this enforcement differs between KT and 6 BA.KT has a significant role in the induction of embryogenic calli at relatively high concentration while 6 BA works well at lower concentration.