基于核酸适体修饰的纳米通道分离β-雌二醇和雌酮的研究

Aptamer-based Au-Nanochannels Membrane for Separation of β-Estradiol and Estrone

建立了修饰金纳米通道分离β-雌二醇和雌酮的新方法。以聚碳酸酯膜为模板,基于模板合成-化学沉积原理,在其表面及膜孔内壁均匀沉积纳米金层,得到一定孔径的金纳米通道,利用扫描电镜(SEM)、透射电镜(TEM)等对其进行研究表征,制备得到均一、可靠的金纳米通道膜。在制备好的金纳米通道表面,通过分子自组装的方式将β-雌二醇核酸适体修饰在金纳米通道内,得到对β-雌二醇具有选择性的纳米通道。β-雌二醇较容易通过修饰后的纳米通道,而雌酮不易通过。考察了β-雌二醇和雌酮在β-雌二醇核酸适体修饰的金纳米通道的迁移特性,以此实现二者的分离。利用50 nm聚碳酸酯膜沉积金3 h,得到孔径约20 nm金纳米通道膜,在0.5 mmol/L Tris-HCl缓冲溶液(pH 7.4)中,进样池浓度为1.76×10!5mol/L的β-雌二醇和雌酮,分离度达到1.76。

A new method for separation of β-estradiol and estrone by the modified gold nanochannels membrane was described. The gold nanochannels were prepared by chemical deposition of gold on polycarbonate templates membrane. The morphology of the nanochannels, characterized by field emission scanning electron microscope （ FESEM ） and transmission electron microscope （ TEM ） , showed that the Au nanochannel wall was smooth and uniform. After being modified by aptamer of β-estradiol using a molecular self-assembly method, the nanochannels membranes facilitated the transportation of β-estradiol through the membranes while the estrone was not easy to permeate. The transport behaviour of β-estradiol and estrone through the gold nanochannels modified with aptamer of β-estradiol was investigated. The results showed that the β-estradiol aptamer modified nanochannels membrane can be used to separateβ-estradiol and estrone based on this unique selective nanochannel membrane. A separation efficiency of 1. 76 can be obtained when the nanochannels with pore size of 20 nm in the medium of pH from 6 . 00 to 8 . 00 .