响应面法优化微生物诱导碳酸钙沉积培养基

Optimization for microbiological-induced CaCO_3 precipitation medium using response surface methodology

通过响应面法对微生物诱导CaCO3沉积的培养基组分进行优化。结果表明,最适宜培养基配方为葡萄糖30g/L、大豆蛋白胨10g/L、尿素50g/L、硝酸钙0.5mol/L、吐温80体积分数0.05%以及氯化镍250μmol/L。葡萄糖浓度和尿素浓度、葡萄糖浓度和硝酸钙浓度、葡萄糖浓度和吐温浓度、硝酸钙浓度和吐温浓度以及尿素浓度和氯化镍浓度,对微生物诱导CaCO3沉淀量的影响有着比较显著的双因子效应。沉淀物CaCO3含有少量有机质,形成方解石型或方解石和球霰石混合晶型的CaCO3,其形貌和堆积密度随外界条件改变而不同。

Response surface methodology was applied to optimize the medium composition for microorganism induced CaCO3 precipitation. The results show that the optimal medium formulation was glucose 30g/L,soybean peptone 10g/L,urea 50g/L,calcium nitrate concentration 0.5mol/L, Tween 80 concentration 0.05%and chlorination nickel 250μmol/L. The glucose concentration and urea concentration,glucose concentration and calcium nitrate concentration,glucose concentration and Tween concentration , calcium nitrate concentration and Tween concentration as well as urea concentration and chlorination nickel concentration had significantly two-factor effect on microorganism induced CaCO3 precipitation yield. Precipitated CaCO3 contained a small amount of organic matter,and the morphology and bulk density of CaCO3 on forming calcite or calcite-vaterite mixed crystal depended on precipitation conditions changes.