AmpFlSTR~ Identifiler~ Plus试剂盒快速直接PCR扩增初探

Rapid and Direct PCR Amplification Using AmpFlSTR~ Identifiler~ Plus Kit

目的探索可应用于法医实践的快速直接PCR扩增方法,以缩短扩增时间,提高STR分型速率。方法联合运用AmpF詛STRR○IdentifilerR○Plus试剂盒与TaKaRa快速PCR检测试剂构建快速直接PCR体系,以FTA血卡为模板,采用优化后的扩增程序在快速PCR仪上进行扩增,分型结果与常规直接扩增方法相比较。结果 AmpF詛STRR○IdentifilerR○Plus试剂盒与TaKaRa的快速PCR检测试剂针对血卡的联合扩增,所得STR分型结果与常规方法一致,扩增用时由常规直接扩增所需的175 min缩短为55 min。结论采用快速直接PCR方法进行扩增,可得到与常规直接扩增一致的DNA分型,扩增时间明显缩短,DNA分型速率大幅提高。

Objective Questing for an approach of rapid and direct PCR amplification that can be applied to forensic practice in order to reduce amplification time and increase STR typing rate. Methods AmpFeSTR（R） Identifiler（R） Plus kit and TaKaRa rapid PCR test reagent were combined and the rapid and direct PCR amplification system was built. With FTA blood cards as tem- plates, the optimized amplification program was adopted to conduct amplification on a fast PCR instrument and then the typing results were compared with those of the conventional direct amplification methods. Results By the combined amplification con- ducted by the AmpFeSTR（R） Identifiler（R） Plus kit with TaKaRa rapid PCR test reagent on blood cards could gain the STR typing results in accordance with the conventional methods＇s. The amplification time was 55min, which was significantly less than the conventional methods＇s 175min. Conclusion Adoption of rapid and direct PCR amplification could obtain the DNA typing results consistent with the conventional methods＇s. The amplification time was significantly reduced and the DNA typing rate was greatly improved.