响应曲面法优化鸦胆子油中油酸甲酯化率的研究

Optimization of Methyl-esterification of Oleic Acid in Brucea javanica Oil by Response Surface Methodology

目的优化鸦胆子油中油酸甲酯化率。方法根据单因素实验结果,选择对鸦胆子油中油酸甲酯化有影响的5个因素:皂化剂浓度、皂化时间、皂化温度、酯化时间、酯化温度;以最佳水平为中心点,设计响应面分析试验,模拟得到优化鸦胆子油中油酸甲酯化率预测模型。结果响应曲面法优化所得条件为:皂化剂浓度0.5 mol/L,皂化温度60℃,皂化时间25 min,酯化温度60℃,酯化时间2 min。结论优化条件下可获得更好的甲酯化率,为气相色谱法(GC)测定鸦胆子油中脂肪酸含量提供了依据。

Objective To optimize the methyl-esterification of the oleic acid in Bruceajavanica oil.Methods Based on the results of single-factor experiments,the predictive mathematical regression model for the optimization of the methyl esterification rate of the oleic acid in was established.Five independent variables,includingh the concentration of saponifier,saponification time and temperature,esterification time and temperature,were selected to design response surface experiment,with the optimum level used as central point.Results The optimized conditions obtained by response surface experiment are as follows：the concentration of saponifier is 0.5 mol/L,the saponification temperature is 60 ℃,the saponification time is 25 min,and the esterification temperature is 60 ℃,the esterification time is 2 min.Conclusion Better esterification rate can be obtained after the optimization of the methyl-esterification conditions,and can provide basis for determination of fatty oil in Bruceajavanica oil by gas chromatography.