龙葵提取物对荷lewis肺癌小鼠肿瘤组织细胞周期调控相关蛋白及凋亡的影响

Effects of Chloroform Extract on Tumor Tissue Cell Cycle Regulation-involved Protein and Apoptosis in Mice with Lewis Lung Tumor

[目的]观察龙葵氯仿及正丁醇提取物对荷lewis肺癌小鼠肿瘤组织细胞周期调控相关蛋白、细胞凋亡及相关凋亡蛋白表达的影响。[方法]建立lewis肺癌模型,64只C57BL/6J小鼠随机分成模型组、环磷酰胺组、龙葵氯仿和正丁醇提取物高、中、低剂量组,给药15d后处死,剥离肿瘤组织,称重,计算肿瘤生长抑制率;TUNEL染色法检测细胞凋亡;免疫组织化学法检测PCNA、p53、p21和Bax、Bcl-2。[结果]龙葵氯仿及正丁醇提取物对荷瘤小鼠肿瘤增殖有一定抑制作用,其中氯仿提取物中剂量组和正丁醇提取物高剂量组的抑瘤率分别为38.93%和32.14%（P〈0.01或P〈0.05）。龙葵氯仿及正丁醇提取物可降低荷瘤小鼠肿瘤组织PCNA和P53阳性细胞表达率,分别为29.7%-58%和32%-56%,并提高P21阳性细胞表达率（20%-32%）（P〈0.01或P〈0.05）。荷瘤小鼠肿瘤组织细胞的凋亡率均不同程度升高（15%-38%）（P〈0.01或P〈0.05）。Bax阳性细胞表达率均有不同程度升高（11%-41%）,Bcl-2阳性细胞表达率则均有不同程度降低（23%-37%）（P〈0.01或P〈0.05）,并可不同程度上调荷瘤小鼠肿瘤组织Bax/Bcl-2的比值。[结论]龙葵正丁醇及氯仿提取物有一定抑制lewis肺癌的生长作用,其作用机制可能与对荷瘤小鼠肿瘤组织细胞周期调控相关蛋白（PCNA、p53、p21）表达的影响、上调肿瘤组织Bax蛋白的表达、下调Bcl-2蛋白的表达,上调Bax/Bcl-2的比值;促进肿瘤组织的细胞凋亡等相关。

[Objective] To observe the effects of mice tumor tissue protein involved in cellcycle regulation, cellapoptosis and apoptosis related proteins expression on lewis lung tumor mice by chloroform extract and n-Butyl acrylate extract from Solanum nigrum L. [Methods] Set up Lewis lung tumor model, 64 mice were randomly divided into a tumor-burdened blank control group,cyclophosphamide group, chloroform and n-butanol extract high, medium and low dose groups, drug delivery 15 days then put them to death, stripping tumor tissue, weighing, calculating tumor growth inhibition rate;TUNEL staining method to detect apoptosis; Immune histochemical method detection of PCNA, p53, p21 and Bax, Bcl-2.[Results] Chloroform and n-butanol extracts from Solanum nigrum L had certain inhibitory effect on tumor proliferation in tumor-burdened mice, the inhibitory rate of chloroform extract medium dose group and n-butanol extract high dose group were respectively 38.93% and 32.14%, two extracts could reduce a tumor-burdened mice tumor tissue PCNA and P53 positive cells expression rate, increase the rate of P21 positive cells expression. The cellapoptosis rate and the Bax positive cells expression rate rose in different extent, the Bcl-2 positive cells expression rate was reduced in different extent, and the extracts could raise the ratio of Bax/Bcl-2 of mice tumor tissues.[Conclusion] The chloroform extract and n-Butyl acrylate extract from Solanum nigrum L has certain inhibition effect on lewis lung cancer growth, the mechanism may be related to a tumor-burdened mice tumor tissue protein involved in cellcycle regulation（PCNA, p53, p21） expression, increasing the influence of tumor tissue expression of Bax protein, cutting the Bcl-2 protein expression, raising the ratio of Bax/Bcl-2, promoting apoptosis of tumor tissue, etc.