降糖药二甲双胍对甲状腺未分化癌细胞增殖及凋亡的作用

Effect of metformin on proliferation and apoptosis of human anaplastic thyroid carcinoma cells

目的:研究二甲双胍对甲状腺未分化癌细胞增殖及凋亡的作用,初步探讨其作用机制。方法:以不同浓度二甲双胍作用于未分化癌SW1736细胞,MTT法检测其增殖,流式细胞术检测细胞周期和凋亡情况。结果:二甲双胍可显著抑制SW1736细胞增殖,其抑制作用呈剂量依赖性,并随着作用时间的延长抑制作用逐渐增强,于72 h达到最大抑制效果。流式细胞检测结果显示二甲双胍可阻滞未分化癌细胞周期至G0/G1期;并可促进肿瘤细胞凋亡,随着二甲双胍作用浓度的递增,肿瘤细胞的凋亡率逐渐增加,20 mmol/L二甲双胍作用48 h后,可使SW1736细胞凋亡率从8.3%增加至13.3%,与对照组相比,有显著统计学差异。结论:二甲双胍可抑制甲状腺未分化癌细胞的增殖活性,阻滞细胞周期进程,诱导细胞凋亡,为甲状腺未分化癌的治疗研究提供了新的方向。

Objective：To investigate the effect of metformin on proliferation and apoptosis of human anaplastic thyroid carcinoma cells, and explore the underlying mechanisms. Methods： Human anaplastic thyroid cancer cell line SW1736 was treated with different doses of metformin. MTF assay was used to detect proliferation viability of cell line. Cell cycle progression and apoptosis were analyzed by flow cytometry. Results：Metformin significantly inhibited the growth of SW1736 cells in time- and dose-dependent manner with a maximal effect at 72 h. The results of flow eytometry showed that anaplastie cancer cell cycle was arrested to G0/G1 phase by metformin. Furthermore,metformin also enhanced apoptosis of SW1736 cells in a dose-dependent manner. Compared with the control group,addition of 20 mmol/L metformin for 48 h increased the percentage of apoptotic cells from 8.3% to 13.3% in SW1736 cells. Conclusion：Metformin can inhibit proliferation and cell cycle progression and induce apoptosis of thyroid carcinoma cell line. Therefore,it may be a potential therapeutic agent for the treatment of human anaplastic thyroid carcinoma.