不同分期老年COPD患者Treg细胞比例及炎性因子水平的研究

The study on the Treg cells proportion and inflammatory factor levels of the elderly patients with COPD in different phases

目的观察和分析不同分期老年慢性阻塞性肺疾病(COPD)患者Treg细胞比例及炎性因子水平的变化情况。方法选取老年COPD患者100例,将处于急性加重期的患者纳入急性组,将处于稳定期的患者纳入稳定组,两组分别纳入42例和58例,选取健康人50例作为对照组。对三组研究对象外周血中的CD4+Foxp3+Treg细胞的比例、CD4+CD25+Treg细胞的比例以及肿瘤坏死因子-α(TNF-α)、白细胞介素-8(IL-8)、白细胞介素-6(IL-6)、白细胞介素-1(IL-1)的水平进行检测和比较。结果急性组患者的CD4+Foxp3+Treg细胞比例显著高于对照组(P<0.05),对照组研究对象的CD4+Foxp3+Treg细胞比例显著高于稳定组(P<0.05);急性组患者的TNF-α、IL-8、IL-6、IL-1水平均显著高于稳定组(P<0.05),稳定组患者的上述指标均显著高于对照组(P<0.05)。结论老年COPD患者在急性加重期表现为Treg细胞比例的异常升高和炎性因子水平的大幅度上升,这可能是造成急性加重期COPD患者感染和损伤程度加重的原因之一,稳定期老年COPD患者仍存在过度炎症反应的现象,但程度相对较低,并出现了Treg细胞比例下降的机体自我保护现象。

Objective To observe and analyze the changes of the Treg cells proportion and inflammatory factor lev-els of the elderly patients with chronic obstructive pulmonary disease （COPD）in different phases.Methods 100 cases of elderly patients with COPD were selected.The patients in the acute aggravating phase were included into the acute group,while the patients in the stable phase were included into the stable group.42 cases and 58 cases were included in-to the two groups respectively.Meanwhile,50 healthy persons were selected as the control group.The proportions of CD4＋ Foxp3＋ Treg cell and CD4＋ CD25 ＋ Treg cell,tumor necrosis factorα（TNF-α）,interleukin-8 （IL-8）,interleukin-6 （IL-6）,interleukin-1 （IL-1）levels in the peripheral blood of the objects in the three groups were detected and com-pared level.Results The proportion of CD4＋ Foxp3＋ Treg cell of the patients in the acute group was significantly higher than that of the control group （P 〈0.05）and the proportion of CD4＋ Foxp3 ＋ Treg cell of the objects in the control group was significantly higher than that of the stable group （P 〈0.05）;the TNF-α,IL-8,IL-6,IL-1 levels of the patients in the acute group were significantly higher than those of the stable group （P 〈0.05）,and the above inde-xes of the patients in the stable group were significantly higher than those of the control group （P 〈0.05）.Conclusion the elderly COPD patients shows the abnormal elevation the Treg cells percentages and the significant increasing of the inflammatory factors in the acute aggravating phase,which may be one of the reasons causing the infection and the damage degree increases of the COPD patients in the acute aggravating phase.There are still excessive inflammation phenomenon in the elderly COPD patients in the stable phase,but the degree is relatively low,and there is the emer-gence of the decreasing percentage of Treg cell of the patients owing to the body self protection.