蟅虫创愈胶囊提取工艺研究

Research on Extraction Process of Zhechong Chuangyu Capsule

目的建立蟅虫创愈胶囊的提取工艺。方法通过小鼠扭体镇痛实验,考察水提工艺和醇提工艺对蟅虫创愈胶囊镇痛作用的影响,确定提取溶剂和工艺。采用正交试验,以出膏率、正丁醇浸出率、川续断皂苷Ⅵ含量为评价指标,以溶剂用量、提取时间、提取次数为因素,考察提取工艺。结果 50%乙醇提取物与水提取物镇痛作用相当,结合生产成本考虑采用水提方法。最佳提取工艺条件为:溶剂用量20倍,提取时间150 min,提取3次。结论该提取工艺切实可行。

Objective To establish the extraction process of Zhechong Chuangyu Capsule. Methods The difference of analgesic effect of water extraction and alcohol extraction in mice was observed by body-torsion test to determine the extract solvent. With the rate of aqueous extraction, n-butyl alcohol extraction and asperosaponin Ⅵ as evaluating indicator, the influencing factors including solvent volume, time and times of extraction were investegated to evaluate extracting procedure by orthogonal experiment. Results There was no obvious difference in analgesic effect between water extraction and alcohol extraction. Given the requirement of produce, aqueous extraction was a better choice. The optimum extracting condition was extracted 3 times with 20 folds volume of solvent, and extraction time was 150 minutes. Conclusion The extraction process is feasible to be applied into production.