CBS mRNA在自发性高血压大鼠脑组织中的表达和分布

CBS mRNA expression and distribution in brain tissue of spontaneous hypertensive rats

目的探讨自发性高血压大鼠(SHR)脑组织胱硫醚β合酶(CBS)的表达及分布情况。方法选择SD(Sprague Dawley)大鼠和SHR各8只,采用逆转录聚合酶链反应(RT-PCR)和real time-PCR技术检测其大脑皮层、小脑、延髓、下丘脑、海马组织CBS mRNA的表达。结果 RT-PCR检测,SHR下丘脑、海马、小脑、大脑皮层及延髓组织的CBS mRNA表达水平分别为0.93±0.01、0.09±0.03、0.36±0.06、0.07±0.01及0.39±0.02,显著低于SD大鼠(P<0.05)。real-time PCR检测,SD大鼠脑组织CBS mRNA的表达水平从高到低依次为大脑皮层、海马、延髓、下丘脑、小脑。SHR脑组织CBS mRNA的表达从高到低依次为下丘脑、延髓、大脑皮层、海马及小脑。SHR脑组织CBS mRNA的表达水平低于SD大鼠(P<0.05)。结论 SHR脑组织中CBS mRNA的表达下调,可能通过中枢机制参与高血压的发病。

Objective To investigate the cystathionine beta-synthase（CBS） expression and distribution in brain tissue of sponta-neous hypertensive rats（SHR） .Methods Eight SD（Sprague Dawley） rats and eight SHR were enrolled .Reverse transcriptase-pol-ymerase chain reaction（RT-PCR） and real time-PCR techniques were adopted to detect the CBS mRNA expression in their cerebral cortex ,cerebellum ,medulla oblongata ,under thalamus ,hippocampus tissue .Results According to RT-PCR ,CBS mRNA expression levels in hypothalamus ,hippocampus ,cerebellum ,cerebral cortex and medulla tissue of SHR were 0 .93 ± 0 .01 ,0 .09 ± 0 .03 ,0 .36 ± 0 .06 ,0 .07 ± 0 .01 and 0 .39 ± 0 .02 ,respectively ,which were significantly lower than those of SD rats （P〈0 .05） .According to real-time PCR ,CBS mRNA expression levels in brain tissue of SD rats from high to low in order were cerebral cortex ,hippocampus ,me-dulla oblongata ,hypothalamus and cerebellum .Those of SHR from high to low in order were hypothalamus ,medulla ,cortex ,hippo-campus and cerebellum .CBS mRNA expression levels in brain tissue of SHR were lower than those of SD rats （P〈0 .05） .Conclu-sion The expression level of CBS mRNA in brain tissue of SHR is down-regulated ,which may be involved in the pathogenesis of hypertension via central mechanism .