水通道蛋白-4抗体阳性患者血清抗Hu、Yo抗体的检测

Anti-Hu,Yo antibodies in patients with positive serum AQP4 antibody

目的观察抗Hu抗体和抗Yo抗体在水通道蛋白-4(AQP4)抗体阳性患者中的出现情况。方法检测收集80例AQP4抗体阳性患者,以间接免疫荧光方法检血清抗Hu抗体、Yo抗体,并回顾性分析抗Hu抗体、抗Yo抗体阳性患者的临床资料及诊断。AQP4抗体检测:先以转染AQP4基因的人胚肾293细胞结合血清中的AQP4抗体,之后采用免疫荧光方法检测;抗Hu抗体、抗Yo抗体检测:先以猴小脑冰冻切片结合血清中的抗体,之后采用免疫荧光法检测。结果 80例患者中,均未检测到抗Hu抗体,仅1例NMO患者血清抗Yo抗体阳性。结论抗Hu抗体和抗Yo抗体在无肿瘤的AQP4抗体阳性患者中少见。

Objective To evaluate the positive rates of serum anti-Hu, and anti-Yo antibodies in patients with positive serum aquaporin-4 （AQP4） antibodies. Methods A consecutive cohort of 80 subjects were analyzed retrospectively. First, sera of patients were tested for AQP4 antibodies in an indirect immunofluorescence assay employing HEK 293 cells transected with recombinant human AQP4. Second, anti- Hu, and anti Yo antibodies were tested with monkey cerebellum substrate by an indirect immunofluoreseenee assay. Results Eighty patients with positive AQP4 antibodies were confirmed in the present study. Only one of the examined sera was positive for anti Yo antibody （1.25%, 1/80） and none was positive for anti-Hu antibodies. This patient suffered from optic neuritis and transverse myelitis, ranging over three vertebral segments on spinal MRI. Conclusions Anti Hu and anti-Yo antibodies were rare in our patients with positive serum AQP4 antibodies.