UHUPLC和HPLC法对比测定不同产地金银花药材中绿原酸的含量

Comparison of UHUPLC and HPLC for Determinations of Chlorogenic Acid in Lonicera Japonica Thunb

目的:用UHUPLC法与HPLC法对比测定金银花药材中绿原酸的含量。方法:分别建立了UHUPLC和HPLC测定金银花药材中绿原酸含量的方法。UHUPLC:色谱柱Agilent ZORBAX RH C18(2.1 mm×50 mm,1.8μm),流速0.2 mL/min,进样量0.5μL;HPLC:色谱柱Agilent ZORBAX XDB C18(4.6 mm×150 mm,5.0μm),流速1.0 mL/min,进样量5.0μL。流动相为乙腈(B)-0.2%磷酸水(A)(13∶87)等度洗脱,检测波长327 nm,柱温30℃。结果:2种方法所得含量测定结果相近。UHUPLC法和HPLC法加样回收率的RSD分别是0.31%和0.33%。结论:UHUPLC较HPLC法更加简便迅速,大大提高了分析的通量、灵敏度、速度,同时减少了有机溶剂的消耗。UHUPLC法可以替代HPLC法,为金银花药材质量控制提供新的检测方法和科学依据。

Objective ： To compare theUHUPLC and HPLC methods for simultaneous determinations of Chlorogenic acid in Lonicera Japonica Thunb. from different areas. Methods ： The UHUPLC and HPLC methods were respectively established for the determinations of Chlorogenic acid in Lonicera Japonica Thunb. UHUPLC was used. The chromatographic separation was performed on a Agilent ZORBAX RH C18 （2. 1mm × 50mm, 1.8μm） column with isoeratie elution. The flow rate was 0.2mL/min. HPLC was used. The chromatographic separation was performed on a Agilent ZORBAX XDB C18 （4.6mm × 150mm,5.0μm） column with isocratic elution. The flow rate was 1.0mL/min. The detection wavelength was 327 nm, the column temperature was 30 ℃. Results ： Both of the two methods for the determination had the similar results. The RSD values of the average recovery of UHUPLC and HPLC were respectively 0.31% and 0.33%. Concluslon..UHUPLC and HPLC methods for the determinations of Chlorogenic acid in Lonicera Japonica Thunb. are simple and the results are accurate and reliable. The major advantages of UHUPLC are the speed of analysis and the narrower peaks. UHUPLC can not only increase separation speed and efficiency, but also reduce solvent analysis time and consumption. UHUPLC as a new detection method can replace HPLC for the quality control of Lonicera Japonica Thunb.