益气健脾抗癌法对肠癌脾虚本质的影响及抗肿瘤作用实验研究

Randomized Parallel Group Study on Impact of Benefiting Qi and Strengthening Spleen and Anti-Colorectal Cancer Herbs on Protein Kinase C and Tumor Epidermal Growth Factor and Vascular Endothelial Growth Factor Expressions

目的:观察益气健脾抗癌中药对脾虚肠癌蛋白激酶C(PKC)及瘤体表皮生长因子(EGF)、血管内皮生长因子(VEGF)表达的影响。方法:使用随机平行对照方法,将40只SD小鼠编号按随机数字表法分为正常组、模型组、益气健脾组、益气健脾抗癌4组。每组10只。灌胃干预:正常组、模型组生理盐水,益气健脾组益气健脾中药,益气健脾抗癌组益气健脾抗癌中药合用。连续灌胃14 d。观察脾脏蛋白激酶C(PKC)、瘤体表皮生长因子(EGF)、血管内皮生长因子(VEGF)表达。结果:脾脏PKC表达正常组高于益气健脾抗癌组、益气健脾组和模型组(P<0.01),正常组、益气健脾抗癌组均高于模型组(P<0.01),益气健脾抗癌组高于益气健脾组(P<0.05),益气健脾组高于模型组(P<0.05)。细胞凋亡益气健脾组、益气健脾抗癌组、模型组均高于模型组(P<0.01),益气健脾抗癌组高于益气健脾组(P<0.01)。结论:益气健脾中药可改善脾功能,缓解肿瘤对脾脏PKC表达抑制作用,益气健脾中药联合抗癌中药作用更加明显,有效促进肿瘤细胞凋亡,对EGF、VEGF表达具有明显抑制作用。

Objective ： Benefiting Qi and strengthening spleen and anti - colorectal cancer herbs on protein kinase C （ PKC ） and tumor epidermal growth factor （EGF） , vascular endothelial growth factor （ VEGF ） expressions. Methods ： Random parallel control method was used. 40 SD mice were randomly divided into normal group, model group, benefiting Qi and strengthening spleen group, benefiting Qi and strengthening spleen and anti - colorectal cancer group, n = 10. The normal saline was given fro model and normal groups. Benefiting Qi and strengthening spleen group and benefiting Qi and strengthening spleen and anti - colorectal cancer group were given corresponding herbs. The treatment course was 14 d. The PKC, EGF, VEGF expressions were observed to determine the efficacy. Results ： After intervention for 14 days, PKC expression in normal group were higher than that in the other groups （P 〈 0. 01 ） and that of normal and benefiting Qi and strengthening spleen and anti - colorectal cancer group was both higher than that of benefiting Qi and strengthening spleen group （ P 〈 0. 01 ）. The PKC expression in benefiting Qi and strengthening spleen and anti - colorectal cancer group was higher than that of benefiting Qi and strengthening spleen group （ P 〈 0. 05 ） and that of benefiting Qi and strengthening spleen group Qi was higher than that of model group （ P 〈 0. 05 ）. The apoptosis in benefiting Qi and strengthening spleen and anti - co]orectal cancer group, benefiting Qi and strengthening spleen group and model group was all higher than that of model group （ P 〈 0. 01 ） and that of benefiting Qi and strengthening spleen and anti - colorectal cancer group was higher than that of benefiting Qi and strengthening spleen group （P 〈 0. 01 ）. Conclusion ：The benefiting Qi and strengthening spleen herbs can improve spleen function as well as relieve the inhibition of PKC and there is more obvious effect when combined with anti - cancer herbs, effectively improving the apoptosis as well as inhibiting EGF and VEGF expressions.