细菌L型变异与临床感染

The relationship of bacterium L-form and clinical infection

目的：研究细菌变异 Ｌ型及检测对临床疾病诊断治疗的意义。方法：用改良 ｋａｇｅｎ氏培养基（用小牛血清代替人血浆），进行细菌Ｌ型培养与普通常规培养对照，用普通血平板加青霉素、高渗液体培养基、胆汁，改良罗氏培养基加链霉素诱导Ｈ３７ＲＶ变异Ｌ型。结果：５７例泌尿系感染患者Ｌ型培养阳性检出率２９．８％，普通培养１２．２％（Ｐ ＜０．０１）。１６５例血 Ｌ型培养检出率 ２６． ７％，普通培养 ９． ０％（ Ｐ＜０．０１ ）。葡萄球菌在血平板加羧苄青霉素玻片在抑菌区内生长出葡萄球菌 Ｌ型。改良罗氏培养基加链霉素诱导 Ｈ３７ＲＶ变异抗酸性颗粒（即 Ｌ型）。细菌 Ｌ型药敏结果与细菌有不同。结论：细菌能变异 Ｌ型，细菌Ｌ型培养阳性检出率明显高于普通培养，Ｌ型菌药敏结果对抗细胞壁类抗生素药物不敏感，对临床疾病的诊治有价值。

Objective: It is to explore the relationship of bacterium L - form variation and the clinical diagnosis of infectious diseases. Methods: In addition to conventional culture media plate, the modified Kagen's medium plate was applied to culture bacteria L - form, and the modified Kagen's medium plate with streptomycin was used for introduce the L - form of Mycobacterium tuberculosis H37RV. Results: In 57 patients with infection in urinary system, the positive rate of bacteria detected in L - form medium and in conventional medium were 29. 8% and 12. 2%, respectively (P < 0. 01 ). In 165 blood samples the positive rate of bacteria detected in L-form and conventional medium were 26. 7 % and 9. 0%, respectively(P <0. 01 ). Conclusion: The positive rates of bactenium in L - form culture medium was significantly higher than that in conventional medium that will benefit the clinical diagnosis in infection diseases.