HPLC法同时测定黄酒中4种非法食品添加剂

Simultaneous Determination of Four Kinds of Illegal Food Additives in Chinese Rice Wine by HPLC

建立高效液相色谱同时测定黄酒中安赛蜜、苯甲酸、山梨酸和糖精钠4种非法食品添加剂的方法。黄酒中的待测物质经提取后,采用C18柱分离,以甲醇–乙酸铵溶液为流动相进行洗脱,在波长230 nm处用高效液相色谱–二极管阵列检测器进行测定。安赛蜜、苯甲酸、山梨酸和糖精钠的质量浓度在0.5～200.0μg/mL范围内与其色谱峰面积的线性关系良好(r>0.999 7),检出限为0.29～0.74μg/L。在0.5,1.0,2.5,5.0,7.5 mg添加水平时的平均回收率在95.6%～104.0%范围内,测定结果的相对标准偏差为0.3%～1.5%(n=6)。该方法操作简便,分离效果好,灵敏度高,结果稳定可靠,适合于黄酒中安赛蜜、苯甲酸、山梨酸和糖精钠的同时测定。

A method of simultaneous determination of four kinds of illegal food additives in Chinese rice wine by high performance liquid chromatography（HPLC）-diode array detector was established. The sample was extracted by water,detection wavelength was 230 nm,ZORBAX Eclipse XDB-C18 was used for chromatographic separation,and methanol-ammonium acetate aqueous solution was used as mobile phase. The mass concentration has good relationship with the chromatographic peak area in the range of 0.5-200.0μg/mL（r〉0.999 7）,and the detection limit was 0.29-0.74μg/L. The relative standard deviations of determination results were 0.3%-1.5%（n=6）. The average recovery rates were 95.6%-104.0%. This method is simple,accurate,sensitive and suitable for the simultaneous determination of illegal food additives in Chinese rice wine.