胃宁颗粒对胃癌前病变基因甲基化的相关调控

The regulation of WeiNing Granules on methylation of gene E-cadherin,P16,c-myc on PLGC

目的通过对模型大鼠胃癌前病变组织中E-cadherin、P16、c-myc基因甲基化的检测,研究胃宁颗粒对胃癌前病变的表观遗传修饰作用。方法采用甲基化特异性PCR(methylation-specific PCR,MSP)方法检测正常组、模型组、治疗组(高、中、低)中E-cadherin、P16、c-myc基因甲基化状态。结果造模24周后,模型组大鼠病理诊断出现肠化生、不典型增生等病理改变。高、中、低治疗组大鼠病理诊断呈不同程度糜烂但较模型组轻。E-cadherin基因各治疗组与模型组比较P>0.0125无统计学差异;各治疗组之间P>0.0125无统计学差异。P16基因高剂量组与模型组比较P>0.0125无统计学差异;中、低治疗组与模型组比较P<0.0125有统计学意义。c-myc基因高、低剂量组与模型组比较P>0.0125无统计学差异;中剂量组与模型组比较,有统计学意义。结论 N-甲基-N-硝基-N-亚硝基胍(MNNG)为主的多因素联合攻击法诱发的大鼠胃癌前病变模型造模成功。胃宁颗粒对E-cadherin基因甲基化无相关作用;对p16、c-myc基因甲基化发生相关调控作用。

Objective To observe the modification effect of WeiNing granules on gastric precancerous lesions by detecting the methylation of gene E- cadherin, P16, c -myc in the tissues of gastric precancerous lesions. Methods All the rat were killed. Methylation- specific technique was used to examine the methylation status of gene E -cadherin, P16, c -myc in control group, model group and treatment group. Results 1.24 weeks after the rat model was built, the rats in the model set were diagnosed to have pathological changes including intestinal metaplasia and atypical hyperplasia. 2. The erosion degree diagnosed with the high, medium and low dosage group was varied, yet its extent is not serious as that of the model set. The comparison of the E - cadherin gene treatment and the model set shows P 〉 0. 0125,which is in good agreement of statistics, and P value has P 〉 0. 0125 that has acceptable variance. P 16 treatment with high dosage compared to the model set has P 〉 0. 0125 showing acceptable variance, and the treatment with medium and low dosage compared to the model set show P 〈 0. 0125 which makes sense from point view of sta- tistics. The c - myc gene treatment with high and low dosage compared to the model set show P 〉 0. 0125 which has acceptable variance and the medium dosage group has which are statistically meaningful. Conclusion 1. The model of precancerous lesions of gastric cancer induced by joint attach method which is mainly depends on the MNNG was successfully built. 2. The Weiing Gran- ules has no effect on the methylation of the E -cadherin gene;However,it has some adjusting effect on the methylation of the P16 and c -myc gene.