苓桂术甘汤对心肌缺血再灌注损伤SOD、MDA含量的影响

Effect of Linggui Zhugan Decoction on SOD,MDA Caused by Myocardial Ischemia-reperfusion Injury

目的:通过观察苓桂术甘汤对心肌缺血再灌注损伤家兔模型超氧化物歧化酶(SOD)活性与丙二醛(MDA)含量的影响,从生物化学水平讨论其防治再灌注损伤的可能机制。方法:40只新西兰大白兔随机分成空白组,模型组,假手术组,苓桂术甘汤高剂量组,苓桂术甘汤低剂量组。采用结扎家兔左冠状动脉前降支的方法制备心肌缺血再灌注损伤模型。缺血40min和再灌注3h后分别采血,检测血清中SOD活性与MDA含量。结果:与模型组比较,高剂量组缺血40min时,SOD活性升高,MDA含量显著降低,再灌注3h时,SOD活性显著升高,MDA含量显著降低;低剂量组缺血40min和再灌注3h时,SOD活性无明显变化,MDA含量显著降低。但总体上高剂量组比低剂量组SOD活性高,MDA含量低。结论:苓桂术甘汤对心肌缺血再灌注损伤具有防治作用,其机制可能与升高SOD活性、降低MDA含量有关,且高剂量组疗效显著优于低剂量组。

Objective： To study the effects of Linggui Zhugan decoction on SOD and MDA caused myocardial ischemia-reperfusion injury in rabbits and explore the mechanism of its possible protection to ischemia-reperfusion injury in the level of biochemistry. Methods： Forty rabbits were randomly divided into blank,model, sham,high-dose,low-dose groups. Myocardial ischemia-reperfusion injury models of rabbits were established by ligating the left descend coronary artery. After ischemia 40 minutes and reperfusion for three hours, examine the amount of SOD and MDA in the serum. Results： Compared with model group after ischemia 40 minutes,SOD in high-dose group was higher,MDA in high-dose group was significantly lower. SOD in high-dose group was significantly higher and MDA was significantly lower after reperfusion for three hours; SOD in low-dose group had no obvious change; MDA was significantly lower after ischemia 40 minutes and after reperfusion for three hours respectively. Compared with low-dose group,SOD in high-dose group was higher and MDA was lower. Conclusion： Linggui Zhugan decoction has a protective effect on myocardial ischemia-reperfusion injury models of rabbits; the mechanism may be related to the increase of SOD activity and to the reduction of excessive production of MDA,and high-dose group is more effective than low-dose group.