慢性应激对大鼠主动脉ET-1、eNOS表达的影响及电针的干预作用

Alteration of ET- 1 and eNOS in Aorta Tissue with Chronicity Stress Depression Model Rats and Effect of Electro- acupuncture

目的:观察慢性应激对SD大鼠主动脉ET-1、eNOS表达的影响以及电针的干预作用,探讨抑郁症与心血管疾病之间的关系。方法:采用慢性应激刺激结合孤养的方式建立大鼠慢性应激抑郁模型,分组后分别采用电针、氟西汀治疗21天,光镜观察大鼠胸主动脉形态学变化,免疫组化法(S-P)测定主动脉ET-1、eNOS表达。结果:HE染色显示,模型组大鼠主动脉组织损伤,针刺干预可以改善此损伤;模型组大鼠主动脉组织ET-1高度表达、eNOS表达降低,电针和氟西汀均可改善变化。结论:慢性应激可引起大鼠的血管内皮结构和功能的损伤,电针和氟西汀可改善这些变化。

Objective： To observe the changes of ET - 1 and eNOS in aorta tissue with chronicity stress depres- sion model rats and the interference of electroacupuncture on them, and to explore the function of the renin - angiotensin system between depression and cardiovascular disease. Methods：The chronic unpredictable mild stress combined with solitarily feeding method was used to make the depression models. The morphological changes of thoracic were observed with light microscope. Immunohistochemistry method was applied to analyze the expres- sion of Endothelin 1 （ ET - 1 ） and Endothelial nitric oxide synthase （eNOS）. Results ： The endothelium of model rats was damaged. Electro - acupuncture could improve those changes. The expression of ET - 1 in the arterial tissue of model rats enhanced. The expression of eNOS in the arterial tissue of model rats reduced. Electro - acupuncture and fluoxetine were obvious to improve those changes. Conclusion：Local tissue RAS is activated excessively in chronicity stress depression model rats. Electro- acupuncture and fluoxetine can improve those changes