溃肠宁对TNBS模型大鼠肠形态、血清及结肠组织IL-6和IL-10影响的观察

Observations on the influences of Kuichangning on morphology,serum and colonic IL-6,IL-10 in TNBS-induced ulcerative colitis rats

[目的]观察中药溃肠宁对三硝基苯磺酸(TNBS)致溃疡性结肠炎(UC)大鼠模型肠黏膜形态、大鼠血清及结肠黏膜组织中白介素-6(IL-6)、白介素-10(IL-10)表达的影响,探讨溃肠宁抗UC的相关作用机制以及疗效。[方法]采用TNBS复合50%乙醇法复制UC大鼠模型,分别采用中药溃肠宁和柳氮磺胺毗啶(SASP)作为阳性对照组进行治疗,对比模型组和空白对照组采用ELISA法对各组大鼠肠血清以及肠黏膜中IL-6、IL-10进行检测,并对其检测结果进行分析。[结果]模型组大鼠结肠和血清中的IL-6含量明显高于正常组(P<0.01),IL-10的含量则明显低于正常组(P<0.01);SASP组结肠及血清中IL-6、IL-10含量与模型组比较差异有统计学意义(P<0.01);中药组和模型组相比,结肠和血清中IL-6含量明显低于模型组(P<0.01),IL-10的含量则明显高于模型组(P<0.01);中药组与西药SASP组比较,中药组结肠和血清中IL-6含量低于西药组,IL-10的含量高于西药组,可见2组之间差异有统计学意义(P<0.01)。[结论]溃肠宁对TNBS复合50%乙醇法UC大鼠疗效显著,其作用机制可能与调节机体的免疫有关。

[Objective]To explore the mechanism and the therapeutic effects of Kui Chang Ning （KCN） against ulcerative colitis （UC） via observation on the morphology of colonic mucosa and the expressions of interleukin-6 （IL-6） and interleukin-10 （IL-10） in serum and in colonic tissue of TNBS induced UC rats.[Methods]UC rat models,established by enteroclysis with three trinitrobenzene sulfonic acid （TNBS） and 50％ othanol,were treated by Kui Chang Ning and SASP and were grouped as positive controls in contrast of the model group and blank control.[Results] ELISA determination on serum and colonic mucosal IL-6 and IL-10 for all groups showed that the serum and colonic IL-6 concentrations in the model group were higher than that in normal group （P＜0.01）,while IL-10 concentrations were lower （P＜0.01）.Significant differences in colonic and serum IL-6 and IL-10 were observed in the SASP group and the model group （P＜0.01）.The KCN group was observed lower serum and colonic IL-6 concentration and higher IL-10 concnetration than the model group （P＜0.01）.When compared with SASP group,KCN group showed significant differences for lower IL-6 concentration and higher IL-10 concentration （P＜0.01）.[Conclusion]The effectivity of KCN on TNBS plus 50％ ethanol induced UC rats may be related to the immune regulation.