IMP1对人乳腺癌细胞增殖的影响

Effect of IMP1 on the proliferation of MDA231 human breast cancer cells

目的观察胰岛素样生长因子ⅡmRNA结合蛋白1(IMP1)的表达对人乳腺癌细胞增殖能力的影响。方法取对数生长期的人乳腺癌细胞株MDA231-IMP1-GFP和MDA231-GFP细胞,用10%胎牛血清DMEM培养液制成相同浓度的细胞悬液,加入培养板中分别培养24、48、72 h后,采用细胞计数板计数法及MTT检测法进行计数及比较。结果 IMP1的表达对细胞形态没有影响。在不同时间点,细胞计数板计数法及MTT法均显示,MDA231-IMP1-GFP细胞的增殖率低于MDA231-GFP(P均<0.01)。结论 IMP1的表达对人乳腺癌细胞MDA231的增殖具有抑制作用。

Objective To investigate the effect of IMP1 on the proliferation of breast cancer cells.Methods Equal amounts of MDA231-IMP1-GFP cells and MDA231-GFP cells were cultured in DMEM medium containing 1 0% fetal bovine serum. Cell number was counted in 24,48,and 72 h with cell counting and MTT methods. The data was statistically analyzed. Results IMP1 had no impact on cellular morphology. In different time point,MDA231-IMP1-GFP cells proliferated more slowly than MDA231-GFP cells by cell counting and MTT methods （P〈0.01 ）. Conclusion IMP1 expression might inhibit the growth of human MDA231 breast cancer cells.