EBV-DNA拷贝数和EBERs原位表达在鼻NK/T细胞淋巴瘤诊断中的一致性研究

A consistency between EBV-DNA copy numbers and EBERs expression contributing to the diagnosis of nasal NK/T-cell lymphoma

目的探讨EB病毒(EBV)-DNA拷贝数和EBV编码的小RNA(EBERs)原位表达在鼻NK/T细胞淋巴瘤(NKTCL)是否具有一致性。方法回顾性分析50例鼻NKTCL的临床病理特征,免疫组织化学检测CD3、CD45RO、CD20、CD56、CD79a、TIA1、Granzyme B表达情况;分别运用实时定量PCR检测石蜡组织中EBV-DNA的拷贝数以及原位杂交检测EBERs,分析与EBV感染与鼻NKTCL的关系。结果鼻NKTCL大部分发生部位位于鼻腔占88%(44/50),伴有出血、坏死以及溃疡者高达100%(50/50)。CD3、CD56、TIA1、Granzyme B及EBV-DNA和EBERs阳性表达率达100%。所有患者的EBV-DNA拷贝数均处于一个较高的水平,最小拷贝数为127.105,最大拷贝数为1 450 000,其中位拷贝数是23 750。结论实时定量PCR检测EBV-DNA拷贝数与原位杂交技术检测EBERs表达具有高度一致性。因此鉴于鼻NKTCL具有组织形态复杂多样性的特点,在诊断鼻NKTCL时需综合考虑临床表现、形态学改变、免疫表型及EBV-DNA或EBERs高水平表达,方能明确诊断。

Objective To explore whether the EBV-DNA copy number and EBERs expression are concordant with nasal NK/T cell lymphoma （NKTCL）diagnosis. Methods 50 patients diagnosed as nasal NKTCL were investigated in this study retrospectively. Expressions of CD3,CD45RO,CD20,CD56,CD79a, TIA1 ,Granzyme B were applied with immunohistochemistry staining. EBV-encoded small RNAs （EBERs） was measured by in situ hybridization,and EBV-DNA copy numbers with realtime quantitative PCR,respectively. Results 88% （44/50）of the nasal NKTCL occurred in nasal cavity. All cases exhibits necrosis,ulceration and bleeding. Expressions of CD3,CD56,TIA1 and Granzyme B as well as high level of EBV-DNA and EBERs were observed in all cases. The median EBV-DNA copy number was shown as 23,750 （the range of 1 27.1 05 to 1 450 000）. Conclusions The high consistency between EBV-DNA copy numbers and EBERs expression was confirmed. Due to its complex morphological diversity,clinical manifestation,pathological changes,and phenotyping with EBV-DNA copy numbers/EBERs expression should be appiled when nasal NK-TCL was considered.