摘要
为观察体外培养绵羊皮肤对外源雌激素加入后的反应,实验对新鲜皮肤、加入雌激素后培养的皮肤和对照组之间通过HE和免疫组化染色的结果进行了比较。结果表明:在HE染色中,加入0.5μg/mL雌激素组和对照组之间没有差异,但是它们与新鲜皮肤相比均出现了皮肤结构的破坏;在免疫组化染色中,加入0.5μg/mL雌激素组皮肤毛囊和皮脂腺普遍出现了凋亡现象,而对照组与新鲜皮肤组则无。表明0.5μg/mL雌激素浓度将导致皮肤毛囊及皮脂腺的凋亡。
This study was investigate to effects of exogenous estrogen on sheep skin cultured in vitro.The skin was divided into three groups：fresh,control and estrogen treated.H＆E and immunohistochemical staining applied to each group.The results showed that,in H＆E stain,there was no difference between 0.5 μg/mL estrogen treated and control,both with skin structure damage compared with fresh skin; in immunohistochemical stain,0.5 μg/mL estrogen initiates apoptosis in hair follicles and sebaceous glands compared with the other two groups.
出处
《中国草食动物科学》
CAS
2014年第3期30-33,共4页
China Herbivore Science
基金
国家绒毛用羊产业技术体系(CARS-40-06)
云南省科技计划项目(2011FB131)
关键词
HE染色
免疫组化染色
毛囊
凋亡
H＆E staining
immunohistochemical staining
hair follicle
apoptosis
