摘要
目的观察针灸"百会"、"肾俞"对阿尔茨海默病(AD)模型大鼠海马神经元线粒体融合蛋白Mfn1、Mfn2的影响。方法采用海马注射Aβ1-42的模型制备方法,将Wistar大鼠随机分为正常组、模型组、假手术组和针灸治疗组,运用免疫组化法观察针灸对线粒体融合蛋白Mfn1、Mfn2的影响。选取单因素方差分析(One-way ANOVA),P<0.05为差异有统计学意义。结果模型组海马神经元线粒体中Mfn1、Mfn2明显低于正常组和假手术组(F=19.075、16.180、12.141、6.287、1.082、9.610、1.502、2.289,P均<0.05),提示在AD发病过程中出现线粒体融合水平下降,线粒体动力学异常;而通过"益肾调督"法针灸治疗以后,可以明显上调Mfn1和Mfn2的水平,改善海马神经元线粒体动力学的异常,与模型组比较差异有统计学意义(F=27.468、19.464、17.459、8.890,P<0.05或<0.01)。结论针灸可通过调节Mfn1、Mfn2的水平达到防止AD的目的。
Objective To observe the influence of acupuncture-moxibustion of‘Baihui＇and‘Shenshu＇on mitofusin-1/2in the hippocampus neurons of rat model with Alzheimer′s disease（AD）. Methods AD rat model was established by injecting Aβ1-42into hippocampus.Wistar rats were divided randomly into normal group,model group,sham-operation group and acupuncture-moxibustion therapy group.The effect of acupuncture-moxibustion on the mitofusin-1/2 was observed by immunohistochemistry.One-way ANOVA was used for data analysis. Results Mfn1and Mfn2fusion protein in mitochondria of hippocampal neurons in rats in model group were significantly lower than those in rats in normal group and sham group（F=19.075, 16.180,12.141,6.287,1.082,9.610,1.502,2.289,P〈0.05）,indicating a declined mitochondrial fusion during AD and an abnormal mitochondrial dynamics.However,after the acupuncture treatment of‘kidney tune Do＇,the levels of Mfn1and Mfn2significantly increased and the mitochondrial dynamics was greatly improved compared with rats in model group,the differences were statistically significant（F=27.468, 19.464,17.459,8.890,P〈0.05,P〈0.05or P〈0.01）. Conclusions Acupuncture-moxibustion can prevent alzheimer′s disease by up-regulating the level of mitofusin-1/2.
出处
《中国预防医学杂志》
CAS
2014年第3期187-190,共4页
Chinese Preventive Medicine
基金
国家自然科学基金(81273864)
