摘要
A novel electrochemical sensor based on the immobilization of tyrosinase（tyr） onto gold nanoparticles（nano-Au） and thioctic acid amide（T-NH2） self-assembled monolayers（SAMs）-modified gold electrode has been developed for the determination of bisphenol A（BPA）.It was found that the nano-Au could significantly enhance the electrochemical response of tyr/nano-Au/T-NH2/Au electrode to BPA,and the enhancement effect of nano-Au on the current response was also related to the enzyme.The results indicated that the biosensor could be used as a detector for BPA determination with a linear range from3.99 ×10-7mol/L to 2.34 ×10-4mol/L and a detection limit of 1.33×10-7mol/L.In addition,this biosensor showed good reproducibility.
A novel electrochemical sensor based on the immobilization of tyrosinase（tyr） onto gold nanoparticles（nano-Au） and thioctic acid amide（T-NH2） self-assembled monolayers（SAMs）-modified gold electrode has been developed for the determination of bisphenol A（BPA）.It was found that the nano-Au could significantly enhance the electrochemical response of tyr/nano-Au/T-NH2/Au electrode to BPA,and the enhancement effect of nano-Au on the current response was also related to the enzyme.The results indicated that the biosensor could be used as a detector for BPA determination with a linear range from3.99 ×10-7mol/L to 2.34 ×10-4mol/L and a detection limit of 1.33×10-7mol/L.In addition,this biosensor showed good reproducibility.
基金
financially supported by the Natural Science Foundation of Hebei Province of China (No. B2010000844)
Research Foundation of Education Department of Hebei Province of China (No. ZH2012078)
