氧化型低密度脂蛋白对小鼠骨髓间充质干细胞迁移的影响

Effect of oxidized low-density lipoprotein on migration of mouse bone marrow-derived stem cells

目的:研究氧化型低密度脂蛋白(ox-LDL)对小鼠骨髓间充质干细胞(bmMSCs)迁移的影响。方法:观察小鼠bmMSCs对ox-LDL的摄取能力。应用不同质量浓度(0、5、10和20 mg/L)ox-LDL诱导培养bmMSCs 6 h后,利用Transwell系统检测bmMSCs跨内皮迁移率,流式细胞仪检测ox-LDL细胞内钙离子阳性率,激光共聚焦显微镜检测细胞骨架F-actin在细胞内的分布。结果:小鼠bmMSCs具有较强的摄取ox-LDL的能力。0、5、10和20 mg/L oxLDL处理组bmMSCs跨内皮迁移率分别为(26.663±1.575)%、(34.653±2.693)%、(38.238±3.050)%和(42.833±3.748)%,钙离子阳性率分别为(42.375±4.281)%、(66.203±5.416)%、(77.958±4.338)%和(84.638±4.768)%,组间比较差异均有统计学意义(F=22.576、63.819,P<0.001)。ox-LDL可促进细胞骨架Factin的重组。结论:ox-LDL可通过调节细胞骨架重组和胞内钙离子阳性率促进bmMSCs的迁移。

Aim： To investigate the effect of oxidized low-density lipoprotein （ ox-LDL） on migration of mouse bone marrow mesenchymal stem cells（bmMSCs）.Methods：bmMSCs were cultured in growth medium with different concentra-tions （0, 5, 10 and 20 mg/L） of ox-LDL for 6 hours.The transendothelial migration rate of bmMSCs was measured using a Transwell system.The distribution of F-actin in bmMSCs was detected using a laser scanning confocal microscope follow-ing Rhodamine phalloidin staining .The concentrations of calcium ion （ Ca2＋） in bmMSCs was measured using a flow cy-tometer following Fluo-3/AM staining.Results： bmMSCs had high potential to take up ox -LDL.The transendothelial migrationrate was （26.663 ±1.575）%,（34.653 ±2.693）%, （38.238 ±3.050）% and （42.833 ±3.748）% in controlgroup, 5, 10 and 20 mg/L ox-LDL groups; there was significant differences among control group and the other 3 ox-LDLgroups（F =22.576,P 〈0.001）.The flow cytometry analysis showed that the positive rate of Ca 2 ＋ was （42.375 ±4.281）%,（66.203 ±5.416）%,（77.958 ±4.338）% and （84.638 ±4.768）% in control group, 5, 10 and 20 mg/Lox-LDL groups; there was significant difference among control group and the other 3 ox-LDL groups（F =63.819,P 〈0.001）.Furthermore, ox-LDL also promoted reorganization of F-actin cytoskeleton.Conclusion： Ox-LDL can promotemigration of bmMSCs via regulation of cytoskeleton organization and Ca 2 ＋ positive rates.