河南省间日疟原虫环子孢子蛋白基因序列分析

Genetic sequence analysis of circumsporozoite protein of Plasmodium vivax from Henan Province

目的：探讨河南省间日疟原虫环子孢子蛋白（ CSP ）的基因型分布。方法：收集经镜检确诊的河南省2011年间日疟患者血标本34份，24例患者为河南籍，发病前无外出到其他省份或国家的历史，10例患者有明确外出史。用核酸提取试剂盒提取滤纸血中疟原虫DNA，进行巢式PCR扩增，对扩增产物进行序列测定和基因进化特征分析。结果：24份本地病例分离所得的本地株间日疟原虫CSP均属于PV-Ⅰ型，又可分为A和B 2个基因型；氨基酸同源性分析结果显示，A型与VK210的同源性达90．6％～94．2％，B型为71．6％。结论：河南省间日疟原虫CSP的基因型均属PV-Ⅰ型，可以进一步分为2个基因型，与其他国家的基因型显著不同。

Aim：To explore circumsporozoite protein （CSP） genotypes structure of Plasmodium vivax（P.vivax） in Henan.Methods：Blood samples of 34 malaria patients from Henan Province in 2011 were collected to extract DNA ,and amplified using nested PCR technique , and the product was sequenced and analyzed .Results：The results showed that the 24 specimens from Henan indigenous cases all belonged to PV-Ⅰ;amino acid homology analysis showed that the specimens could be divided into two genotypes：A and B, the sequence of type A showed 90.6%-94.2%homology to VK210, while type B showed 71 .6%.Conclusion：The P.vivax CSP genotype of Henan all belongs to PV-Ⅰ, and it can be further di-vided into two genotypes , which is significantly different from other countries .