人参皂甙Rb1对感染性休克大鼠重要器官的保护作用

Protective effect of ginsenoside Rb1 on septic shock rats

目的:探讨人参皂甙Rb1对感染性休克大鼠重要器官的保护作用及其机制。方法:66只SD大鼠随机分为假手术组、模型组、Rb1治疗(0.04 mg/g)组,每组22只。模型组和Rb1治疗组大鼠建立感染性休克动物模型。观察3组大鼠肝、肺组织病理变化,检测血清肿瘤坏死因子-α(TNF-α)含量和心肌组织Toll样受体4(TLR4)mRNA表达的变化。结果:模型组肝细胞肿胀、变性、坏死,中性粒细胞浸润;Rb1治疗组肝细胞浊肿,轻度变性,未见明显坏死灶,肝血窦中中性粒细胞浸润较轻。模型组部分肺泡上皮细胞肿胀,肺泡隔增宽,毛细血管明显扩张淤血,微血栓形成,肺间质充血水肿,弥漫性中性粒细胞浸润,肺泡腔内大量炎性渗出;Rb1治疗组小血管及毛细血管内中性粒细胞浸润较轻,肺间质轻度水肿,肺泡隔增宽不明显,肺泡腔无渗出物。模型组血清TNF-α、心肌组织TLR4mRNA水平高于假手术组,Rb1治疗组两指标低于模型组(P<0.01)。结论:Rb1可保护感染性休克大鼠肝、肺组织,其机制可能与减少TLR4 mRNA表达水平,抑制TNF-α的产生有关。

Aim：To observe the protective effect of ginsenoside Rb 1 on vital organs in rats with septic shock .Meth-ods：The septic shock model was reproduced by CLP .Sixty-six SD rats were randomly divided into three groups （ 22 in each group）：sham group, CLP group and ginsenoside Rb1 group（0.04 mg/g）.The liver and lung samples were prepared for observation of pathological changes;the expression of Toll like receptor 4（TLR4） mRNA in myocardial tissue was deter-mined by RT-PCR;the serum tumor necrosis factor-α（TNF-α） was measured by ELISA.Results：In CLP group, patho-logical results showed swelling , degeneration , necrosis and neutrophil infiltration in the liver cells , which were milder in ginsenoside Rb1 group.Moreover, in CLP group, there were swelling in alveolar epithelial cells , thickening in alveolar septum, expansion and congestion in capillaries , microthrombosis, congestion and edema in pulmonary interstitial , diffuse infiltration of neutrophils and diminished alveolar space with heavy inflammatory exudation ,which were milder in ginsen-oside Rb1 group.The TLR4 mRNA in myocardial tissue and serum TNF-αin CLP group were significantly higher than those in sham group（P〈0.01）.The ginsenoside Rb1 group had markedly lower TLR4 mRNA expression and TNF-αlevel than the CLP group（P 〈0.01）.Conclusion： Ginsenoside Rb1 could protect the liver and lung tissue of septic shock rats through down-regulating the expression of TLR 4 mRNA and inhibiting the produce of TNF-α.