糖脂平对胰岛素抵抗大鼠糖脂代谢的影响

Effects of Tangzhiping on Glucose and Lipid Metabolism in Insulin-Resistance Rat Model

目的:建立喂养型胰岛素抵抗(IR)大鼠模型,观察糖脂平对喂养型IR大鼠糖脂代谢的影响。方法:选用体重在180～200 g的8周龄雄性SD大鼠60只,适应性喂养1周后,随机分为空白对照组15只和造模组45只。空白对照组采用普通饲料喂养,造模组采用高脂饲料喂养。8周造模成功后,将造模组随机分为:模型组、糖脂平组、罗格列酮组各15只。糖脂平组给予糖脂平按生药20 g/(kg·d)灌胃;罗格列酮组给予罗格列酮0.8 mg/(kg·d)灌胃;模型组和空白对照组灌服等量的生理盐水;末次给药后禁食12 h,用正常血糖胰岛素钳夹技术来评价胰岛素敏感性,并检测各组大鼠的体重、血糖、血清胰岛素、血脂(TG、TC、HDL-C、LDL-C)等指标。结果:糖脂平能够增加胰岛素敏感性,改善胰岛素抵抗,抑制体重增长,效果优于罗格列酮;同时可降低血糖、空腹血清胰岛素,降低TG和LDL-c,与罗格列酮药效相近。结论:糖脂平能够调整IR大鼠的糖脂代谢紊乱,改善胰岛素抵抗。

Objective：To investigate the effects of Tangzhiping on glucose and lipid metabolism in rats with insulin resistance .Meth-ods：Sixty male SD rats which were 8 weeks old and weighted among 180 to 200 g were randomly divided into blank control group （ n=15） and module group （n=45） after being fed for a week for adaptation .Rats in the blank control group were fed with common food , while module group fed the rats with high fat food for 8 weeks.Eight weeks later, the model rats were successfully made , and they were randomly divided into：the model group （n=15）, Tangzhiping group （n=15）, rosiglitazone group （n=15）.Tangzhiping group gave the rats Tangzhiping formula particles according to the statement 20 g/kg/d to fill in the stomach;rosiglitazone group applied up to 0.8 mg/kg/d rosiglitazone gavage;model group and blank control group used the same amount of saline solution for 8weeks.Twelve hours after the last administration , levels of insulin sensitivity was evaluated by the “euglycemic hyperinsulin clamp technique”, and levels of body weight, fast plasma glucose, serum insulin, blood lipids were also observed .Results：Tangzhiping could raise insulin sensitivity , improve IR, suppress weight gain which was better than rosiglitazone;it could lower blood sugar, reduce serum insulin, TG and LDL-c, which was close to the efficacy of rosiglitazone .Conclusion： IR model is successfully established .Tangzhiping and rosiglitazone can lose weight , reduce blood sugar and insulin levels , adjust blood lipid levels and increase insulin sensitivity .