反相高效液相色谱法测定甜叶菊中A苷甜菊糖和甜菊苷含量

Determination of Content of Rebaudioside A and Stevioside in Stevia rebaudiana Bertoni by RP-HPLC

采用超声辅助提取，建立甜叶菊中A苷甜菊糖和甜菊苷的高效液相色谱分析方法。采用Kroma-sil C18（4．6mm×250mm，5μm）色谱柱，以甲醇：水（75：25，V／V）为流动相，流速1．0mL／min，检测波长210nm，柱温30℃。A苷甜菊糖进样量在0．868～8．68μg时，与峰面积呈良好的线性关系（R=0．999），平均回收率为97．5％，RSD为1．8％（n=6）；甜菊苷进样量在1．004-10．04μg时，与峰面积呈良好的线性关系（R=0．999），平均回收率为95．6％，RSD为1．6％（n=6）。该方法准确、操作简便，可用于甜叶菊中A苷甜菊糖和甜菊苷含量测定。

HPLC method for the determination of rebaudioside A and stevioside in Stevia rebaudiana Bertoni is established by ultrasonic assisted extraction method. The separation is achieved on a Kromasil C18 column （4.6 mm×250 mm, 5 μm） at 30 ℃ using methanol and water solution （75 ： 25, V/V） as the mobile phase at a flow rate of 1.0 mL/min, 210 nm as the detection wavelength. Rebaudioside A shows good relationship at the range of 0. 868-8.68 μg （R=0.999）, the recovery is 97.5% and RSD is 1.8% （n=6）. Stevioside shows a good relationship at the range of 1. 004-10.04 μg （R= 0. 999）, the recovery is 95.6% and RSD is 1.6% （n=6）. The proposed method is accurate, simple and applicable for the determination of rebaudioside A and stevioside in Stevia rebaudiana Berton.