贵州省苗族、布依族及汉族EB病毒感染情况及鼻咽癌诊断试验效果评价研究

The investigation of EB virus infection of Miao,Buyi and Han population and evaluation of the diagnosis of Zta-IgA,EBNA1-IgA and VCA-IgA antibody for nasopharyngeal carcinoma in Guizhou

目的调查贵州省苗族、布依族及汉族EB病毒感染情况并探讨EB病毒特异性ZtaIgA、EBNA1-IgA与VCA-IgA抗体定量检测对鼻咽癌的诊断价值。方法采用随机抽样的方法抽取苗族、布依族及汉族共3040例研究对象,收集研究对象的基本信息;采用ELISA法检测研究对象血浆EB病毒的Zta-IgA、EBNA1-IgA、VCA-IgA标志物水平,分析上述指标诊断鼻咽癌的价值。结果 3040例研究对象中,Zta-IgA阳性率为15.03%;EBNA1-IgA阳性率为16.09%;VCA-IgA阳性率为14.41%(χ2=16.027,P=0.000);Zta-IgA、EBNA1-IgA、VCA-IgA阳性率均为布依族最高(P<0.05);>45岁组Zta-IgA、EBNA1-IgA、VCA-IgA阳性率均明显高于≤45岁年龄组(P<0.05);吸烟、饮酒可以明显提高Zta-IgA、EBNA1-IgA、VCA-IgA阳性率(P<0.05);三者联合检测对诊断鼻咽癌的灵敏度和特异度均明显升高。结论贵州省少数民族EB感染率水平较高,应开展Zta-IgA、EBNA1-IgA、VCA-IgA监测;三者联合检测对鼻咽癌的早监测、早发现有积极意义。

Objective To investigate EB virus infection of Miao, Buyi and Han population and evaluate the diagnosis of Zta-IgA, EBNAI-IgA and VCA-IgA antibody for nasopharyngeal carcinoma in Guizhou Province. Methods A random sampling method was used to select 3040 subjects from Miao, Buyi and Han, collecting basic information. ELISA was used to detected serum Zta-IgA, EBNA1- IgA, VCA-IgA of EB virus for analysing the value of these indicators diagnosis of NPC. Results Zta- IgA positive rate was 15.03%. EBNAI-IgA -positive rate was 16.09%. VCAI-IgA -positive rate was 14.41~ （X2 ----- 16. 027, P~ 0. 000）. Zta-IgA, EBNAI-IgA , VCA-IgA-positive rate of Buyi wasthe highest （P^0.05）. Zta-IgA, EBNAI-IgA, VCA-IgA -positive rate of ~ 45 age group was signifi- cantly higher than that of ~ 45 age group （P〈0.05）. Smoking, alcohol could significantly increase Zta-IgA, EBNAI-IgA, VCA-IgA -positive rate （P〈0.05）. Combined detection of Zta-IgA, EBNA1- IgA, VCA-IgA could significantly increase the sensitivity and specificity for diagnosis of NPC. Conclu- sion EB prevalence of minority in Guizhou is high and Zta-IgA, EBNAI-IgA, VCA-IgA monitoring should be carried out; There is positive significance of combined detection of three to the early monitoring, early detection of nasopharyngeal carcinoma.