七氟烷对新生大鼠海马胶质纤维酸性蛋白表达的影响

Influence of expression sevoflurane inhalation of newborn rat hippocampal glial fibers acidic protein(GFAP)

目的探讨吸入2.3%七氟烷对新生大鼠海马胶质纤维酸性蛋白(GFAP)水平和远期学习记忆功能的影响。方法 7d龄SD大鼠24只,随机分为对照组和七氟烷组,每组12只。对照组持续吸入氧气6h,氧浓度29%;七氟烷组持续吸入2.3%七氟烷6h,氧浓度29%。停止吸氧和七氟烷后4h,每组随机断头处死6只,提取海马组织,蛋白免疫印迹法测定GFAP水平。其余每组6只饲养至14d时行跳台试验。结果与对照组比较,七氟烷组海马GFAP表达明显减少(P<0.05)。跳台试验中,七氟烷组反应时间长于对照组,错误次数也明显多于对照组,七氟烷组潜伏时间短于对照组(P均<0.05)。结论新生期大鼠七氟烷可影响远期学习记忆功能,该效应可能与新生期海马内GFAP表达减少有关。

Objective To explore the 2.3% sevoflurane inhalation of newborn rat hippocampal glial fibers acidic protein （GFAP） and the influence of the long-term learning and memory function. Methods Total of 24 SD rats of 7 day age were randomly divided into control group and sevoflurane group （n=12 for each）. The control group was continuous oxygen 6 h with 29% oxygen concentration. Sevoflurane group was sustained 2.3% sevoflurane inhalation 6 h with 29% oxygen concentra- tion. After stopped oxygen and sevofluran, the samples were randomly beheaded to death at the num- ber of 6 in each group, then extracted the hippocampus and tested the level of GFAP by western blot method. The rest of the each group was to be raised until 14 days to be experimented on the platform. Results Compared with control group, Sevoflurane group hippocampal GFAP expression decreased sig- nificantly （P〈0.05）. Diving platform test, sevoflurane group reaction time was longer than the con- trol group, and wrong number also significantly greater than the control group, and sevoflurane group lurking shorter than the control group （P〈0.05）. Conclusion Neonatal rat sevoflurane can affect the long-term learning and memory functions, this effect may be associated with the decreases of GFAP in the newborn hippocampus.