中药配伍对血清阿魏酸临床生物利用度的影响

Effect of Combination of Chinese Herbal Drugs on Clinical Bioavailability of Ferulic Acid in Serum

目的 :研究中药配伍对方剂吸收入人体内成分生物利用度的影响。方法 :健康自愿者一次口服川芎汤 ( 1g/kg)、川芎芍药汤 ( 2g/kg ,两药之比为 1∶1)、冠心Ⅱ号汤 ( 3g/kg ,其中川芎为 0 5g/kg)后 ,用建立的HPLC法测定吸收入循环血中的阿魏酸 (FA)血药浓度—时间曲线 ,用梯形法计算血药浓度—时间曲线下面积 (AUC) ,然后比较AUC值。结果 :3组的血药浓度时间曲线均为双峰现象。与川芎汤的AUC〔( 15734 88± 7737 97)ng/ (min·ml)〕比较 ,川芎配伍芍药后AUC〔( 5717 92± 612 4 63)ng/ (min·ml)〕值明显降低 (P <0 0 5) ,而川芎配伍在冠心Ⅱ号 (川芎的剂量仅为川芎汤的 50 % )中的AUC〔( 80 72 0 0±4 4 2 4 31)ng/ (min·ml)〕相对不低。结论 :冠心Ⅱ号中药不影响FA的生物利用度 。

To observe the effect of combination of Chinese herbal drugs on bioavailability of Ligusticum walichii (LW) derived ferulic acid (FA) in serum. Methods: Healthy volunteers were given LW decoction (1g/kg), LW combined with Paeonia veitchii (PV, 1∶1) decoction (2 g/kg) and Coronary Heart No.2 (CH Ⅱ, 3 g/kg, containing LW 0.5 g/kg) once respectively. The serum FA concentration time (c t) curve was performed by HPLC using the method established by the authors, and the area under that curve (AUC) was calculated and compared. Results: The FA c t curve in the three groups was double peak curve. As compared with the AUC in subjects who were administered LW decoction, the AUC in those taken LW+PV decoction was lower 〔(15734 88±7737.97) ng/(min·ml) vs (5717 92±6124 63) ng/(min·ml)〕, P<0 05), while that in those taken CH Ⅱ was relatively higher 〔(8072.00±4424.31) ng/(min·ml),P>0 05)〕. Conclusion: Combination of LW and PV could significantly lower the bioavailability of FA, but when LW was added in CH Ⅱ, the FA bioavailability was not affected.