补肾固髓片治疗多发性硬化的临床与实验研究

Clinical and Experimental Study on Multiple Sclerosis with Bushen Gusui Tablet

目的 :观察补肾固髓片对多发性硬化 (MS)的疗效及对实验性变态反应性脑脊髓炎 (EAE)豚鼠的影响。方法 :应用补肾固髓片治疗MS患者 4 3例 ,观察其治疗前后临床症状、神经功能体征、复发次数及诱发电位、磁共振 (MRI)等变化 ;采用兔脊髓匀浆和完全福氏佐剂诱导豚鼠EAE动物模型 ,应用补肾固髓片治疗 ,以醋酸泼尼松作为对照药物 ,观察其发病、神经组织病理变化及其血清白细胞介素 2 (IL 2 )、白细胞介素 6(IL 6)、肿瘤坏死因子 (TNF)和髓鞘碱性蛋白 (MBP)水平。结果 :补肾固髓片可改善MS患者的病情 ,减少复发 ,总有效率 88 37% ;实验研究发现大剂量补肾固髓片能明显抑制EAE发病 ,抑制脑和脊髓的炎症反应和脱髓鞘改变 ,抑制血清IL 2、IL 6、TNF和MBP的活性 (与模型组比较 ,P <0 0 1) ,与醋酸泼尼松组比较差异无显著性 (P >0 0 5)。结论 :补肾固髓片治疗MS疗效较好 。

To observe the therapeutic effect of Bushen Gusui tablet (BSGS) in treating multiple sclerosis (MS) and its effects on experimental allergic encephalomyelitis (EAE) in guinea pigs. Methods: Forty-three MS patients were treated with BSGS and their clinical symptoms, signs of nerve function, recurrent frequency, evoked potential and changes in magnetic resonance imaging (MRI) were observed. The EAE model of guinea pigs was induced by homogenate of rabbit spinal cord and complete Freund′s adjuvent (CFA), the animals were treated with BSGS and compared with prednisone acetate, which was served as control. The mortality and pathomorphology of EAE animals were observed. The contents of serum interleukin-2 (IL-2), interleukin-6 (IL-6), tumor necrosis factor (TNF) as well as myelin basic protein (MBP) were determined. Results: BSGS could improve symptoms and signs of MS patients and reduce recurrent frequency. The total effective rate was 88.37%. High dose BSGS could obviously reduce incidence of EAE, inhibit inflammatory reaction of brain and spinal cord as well as demyelination, and simultaneously inhibit the activity of serum IL-2, IL-6, TNF and MBP, in comparing with model group (P<0 01). There were insignificant difference as compared with prednisone acetate group (P>0 05). Conclusion: BSGS had certain effect on both MS patients and EAE model animals, which indicated that it was worth further studying and clinical application.