c-myc基因信使核糖核酸在异种移植超急性排斥反应中的表达

mRNA Expression of c-myc Gene in the Hyperacute Rejection of Xenotransplantation

目的 :研究 c- myc基因在异种移植超急性排斥反应时的表达及功能。 方法 :应用离体大鼠心脏人血浆体外灌注的异种移植超急性排斥反应模型 ,同时应用分子原位杂交技术进行 c- myc基因信使核糖核酸 (m RNA)在超急性排斥反应中的表达及细胞定位。 结果 :超急性排斥反应后 c- myc基因 m RNA在心脏血管内皮细胞表达升高 ,实验组阳性信号值为 45 0 .0 9± 40 9.99,对照组信号值为 174.40± 5 1.5 0 (P<0 .0 5 )。 结论 :在异种移植超急性排斥反应中 ,c- myc基因表达升高可能与血管内皮细胞活化有关。

Objective:To research expression and function of c myc gene in hyperacute rejection of xenotransplantation. Method:Through the model of hyperacute rejection of xenotransplantation with isolated rat heart perfused with human plasma,we detected c myc mRNA expression and cell localization with in situ hybridization in hyperacute rejection. Result:c myc mRNA increased expression in the endothelium cell of heart.The value of signal is 450.09±409.99.The value of signal of group of control is 174.40±51.50( p <0.05)。 Conclusion:From these study findings,it would appear that hyperacute rejection is associated with the elevation of c myc mRNA expression.