胚胎脊髓移植加神经生长因子和尼莫地平对成年大鼠脊髓继发性损伤的影响

EFFECT OF FETAL SPINAL CORD GRAFT WITH NERVE GROWTH FACTOR AND NIMODIPINE IN SECONDARY INJURY OF SPINAL CORD OF ADULT RAT

目的 用神经生长因子 (NGF)和尼莫地平 (ND)增强胚胎脊髓移植修复成年大鼠损伤脊髓的效果 ,缓解脊髓继发性损伤。方法 采用脊髓半切洞损伤模型 ,分为单纯胚胎脊髓移植组 ,移植加 NGF组 ,移植加NGF和 ND组。术后观察移植部位脊髓细胞内游离钙离子浓度 ,超氧化物歧化酶 (SOD) ,丙二醛 (MDA)的变化 ,并比较各组死亡率。结果 移植加 NGF组较单纯移植组 ,MDA明显下降 ,SOD显著升高 ,有统计学意义 (P<0 .0 5 ) ,但两组间细胞内游离钙离子浓度差别不显著 (P>0 .0 5 ) ;移植加 NGF和 ND组更有效地升高 SOD浓度 ,降低钙离子浓度和 MDA含量 ,与其它两组比较有显著性差异 (P<0 .0 5 ) ;移植加 NGF和 ND组的死亡率明显低于其它两组 ,有非常显著性差异 (P<0 .0 1)。结论 NGF和 ND能较好地改善继发性脊髓损伤 ,明显降低经胚胎脊髓移植术后的成年大鼠死亡率。

Objective To observe the effect of nerve growth factor (NGF) and nimodipine (NP) on fetal spinal cord graft in repair of injury of spinal cord. Methods A total of 144 adult Wistar rats were included in this study. All were made as the hemi-section cavity injury model at the lumbar enlargement and divided into three groups: fetal spinal cord graft (group Tr), fetal spinal cord graft with NGF (group TN), and fetal spinal cord graft with NGF and NP (group TNN). The intracellular concentration of free ionic calcium was measured at the 4th, 8th, and 24th hour, and superoxidase (SOD) and malondialdehyde (MDA) at 3rd, 6th, 12th, 24th and 72nd hour after operation. Results After spinal cord was injured, the concentration of MDA and intracellular concentration of free ionic calcium increased and reached to the peak at the 6th and 8th hour respectively, but SOD decreased and at 24th hour to its vale. The MDA was significantly lower in group TN than in group Tr, while the SOD was higher ( P< 0.05). There was no significant difference on intracellular free ionic calcium concentration between group Tr and TN. The concentration of SOD of group TNN was the highest and the intracellular concentration of free ionic calcium was the lowest in the three groups ( P< 0.05). The weekly mortality was 33%, 31%, 17% respectively in group Tr, TN and TNN. The mortality of group TNN was significantly lower than the other two groups ( P <0.01). Conclusion Although the fetal spinal cord graft is an effective method to repair laboratory spinal cord injury, NGF and ND can interrupt secondary injury and increase survival rate of the host.