纤维堆囊菌接种量对埃博霉素产量的影响

The impact of inoculation quantity on the production of epothilone in Sorangium cellulosum So 2161

埃博霉素是粘细菌纤维堆囊菌产生的一种具有抗肿瘤活性的十六元大环内酯类化合物。纤维堆囊菌有聚团生长的特性,它的生长具有一定的密度依赖性.本文研究了纤维堆囊菌So2161菌体的接种量与埃博霉素产量的关系。结果表明在一定范围内埃博霉素产量随接种量的增加而增加,接种量超过一定值后埃博霉素产量不再增加反而会有一定程度的减少,说明菌体在某个密度时菌体生长最佳,有利于埃博霉素的合成。这为大规模生产埃博霉素提供了菌体密度的参考依据。

Epothilones, which are macrocyclic lactone anticancer compounds that naturally produced by the myxobacterium Sorangium cellulosum. The ceils of Sorangium cellulosum are easy to aggregate together, and have the density-dependent character. In this paper we studied the impact of inoculation quantity on the production of epothilone, and the result was that when the quality was under some value, the production of epothilone increased with the inoculation quantity, but when the quantity exceeded some specified limit, the production of epothilone would not increase and even decrease in a way. This result indicated that the Sorangium cellulosum grow well and the epothilone reached their highest production in a specified density, this will provide reference for large scale production of epothilone.