豚鼠血管内皮细胞培养的研究

STUDY ON CULTURE OF GUINEA PIG'S VASCULAR ENDOTHELIAL CELLS IN VITRO

目的 :建立稳定的豚鼠血管内皮细胞原代及传代培养方法 ,并保证豚鼠取材后较高的存活率。方法 :采用自豚鼠一侧颈总动脉取材进行血管内皮细胞培养 ,克服了以往小动物血管内皮细胞培养自大血管取材 ,取材后动物均死亡的缺点。由于颈总动脉管腔细小 ,原代培养获取细胞数少 ,培养不易成功。采用新的血管外翻方法进行酶消化获取内皮细胞 ,并创造有利于细胞生长的最适条件。结果 :血管内皮细胞扩增迅速 ,纯度较高 ,为进一步研究奠定了基础。结论 :自豚鼠颈总动脉取材进行血管内皮细胞培养能够获得令人满意的结果。

Objective: The study was designed to obtain abundant supply of high-purified Guinea Pig's vascular endothelial cells and ensure high survival rate of Guinea Pigs after operation for the subsequent study. Methods: The vascular endothelial cells was taken from one side of Guinea Pig' common carotid artery and a new method was used to turn the endangium outside and incubated with warmed 0.1% collagenase. The optimal circumstance was also studied to the benefit of endothelial cells growth. Results: The amounts of high-purified vascular endothelial cells can be increased in a short period and be used in the subsequent study. Conclusion: This new method of isolation and culture of endothelial cells is satisfactory.