缺血性脑损伤大鼠下丘脑c-fos基因表达及药物调控

C-FOS GENE EXPRESSION AND MEDICINAL REGULATION IN HYPOTHALAMUS OF RAT FOLLOWING ISCHEMIC BRAIN INJURY

目的 :从分子水平探讨缺血性脑损伤的病理生理改变和参麦注射液的保护作用。方法 :将 15只雄性wistar大鼠随机分为对照组、缺血组和治疗组 ,采用免疫组织化学方法观察了缺血性脑损伤时大鼠下丘脑视上核和室旁核内神经元c -fos基因的表达及参麦注射液的保护作用。结果 :缺血性脑损伤时大鼠视上核和室旁核内神经元c -fos基因的表达明显增多 (P <0 .0 5 ) ,而用参麦注射液后明显减少 ,但未减少至正常水平 (P <0 .0 5 )。结论 :缺血性脑损伤时下丘脑中c -fos基因表达增多 ,而参麦注射液对缺血性脑损伤可能有一定治疗作用。

Objective: To explore the patho-physiologic machanism and the protective role of Shenmai injection following ischemic brain injury at the molecular level, and to provide some theoretical guidance for clinical pathologic study and treatment. Methods: 15 male Wistar rats were divided into control group, ischemia group and treatment group at random. C-fos gene expression and the protective role of Shenmai injection were studied in hypothalamic supraoptical nucleus(SON) and paraventricular nucleus(PVN) following ischemic brain injury with immunohistochemical techniques, Results: In all rats following ischemic brain injury, c-fos gene expression was increased obviously in SON and PVN compared with normal rats, and it was decreased with the application of shenmai injection, but still higher than normal c-fos gene expression in hypothalamus. Conclusion: C-fos gene expression in hypothalamus increases after ischemic brain injury and Shenmai injection can cure the ischmic brain injury in a way.