脑损伤及胎脑移植的实验研究

Experimental study of brain injury and embryonic brain tissue transplantation in rats

目的 观察胎脑细胞悬液延迟移植于脑损伤的生长情况。 方法 46只SD大鼠采用自由落体撞击法制备脑损伤模型。 16只仅做单纯脑损伤 ,伤后 14,2 0 ,30 ,40d处死大鼠做HE染色形态学观察 ;另 30只做胎脑移植 ,于伤后 14d做同种胎脑经机械分离的细胞悬液延迟移植 ,移植后 1,5 ,14,30 ,75d处死大鼠做HE染色形态学观察。 结果 所有实验大鼠生长良好 ,无肢体偏瘫。胎脑细胞悬液浓度为 4.48× 10 7/ml,活细胞占 85 .3 % ,神经细胞占 48.2 %。伤后 14d表现为陈旧性损伤 ,随时间推移 ,创伤灶被胶质细胞充填。移植后 5d出现免疫细胞 ,14d胎脑细胞存活 ,宿主的毛细血管、胶质细胞增生 ,30d胎脑细胞发生分化 ,75d胎脑细胞与宿主相互融合。免疫细胞和小部分移植物碎片同时始终存在。 结论 大鼠脑损伤后行胎脑细胞悬液移植 ,移植物是可以存活并可能发挥功能的。

Objective To demonstrate morphologic features of the cell suspension of the embryonic brain tissues which were transplanted into lesion cavities of brain injury models. Methods In 46 SD rats, cerebral contusion models were made by dropping weight method (impact energy: 20 g×30 cm); of which 16 rats were sacrificed at 14, 20, 30, 40 days after injury and their lesion brains under HE staining, the other 30 rats transplanted by embryonic tissue homogenate at 14 days after injury. The grafted rats were sacrificed at 1, 5, 14, 30, 75 days after implantation and their brains stained by HE dye. Results All experimental rats survived and no hemiplegia seen. The concentration of fetal brain suspension was 4.48×10 7 cells/ml. The living cell was 85.3% and neurocyte 48.2%. After 14 days posterior to injury, the main features under microscope was glia proliferation and scar tissue. The immunocytes were found 5 days after transplantation; the grafts survived and the capillary and the glia proliferation in host were noted 14 days later. Some neurofibers were derived from cells within the grafts 30 days after transplantation. The graft was well integrated into host at 75 days posterior to implantation. Conclusions The donor cells can be survived and influence host and grafts itself.