枣组培苗试管外微嫁接技术研究

Study on the Outside Tube Micrografting Techniques for the Tissue Cultured Zizpihus jujube Buds

以高5～7cm无根健壮的灵武长枣组培苗为接稳，以酸枣实生苗为砧木，采用枣树组培苗试管外微嫁接技术，研究了嫁接口的不同保湿材料、组培苗练苗时间、砧木苗龄、不同浓度BA对嫁接成活率的影响。结果表明：采用封口膜缠绕嫁接口操作容易，保湿效果显著；组培苗最适宜的练苗时间为5～6d；2片真叶苗龄的砧木嫁接成活率高；最适宜的BA浓度是15mg／L；获得最高嫁接成活率是91．4％。

Taking 5-7 cm and no root healthy tissue culture seedlings of‘Lingwuchangzao＇ as scion and Zizyphus jujube seedlings as the rootstock, using the outside tube micrografting techniques, the effect of different moisture materials,time of hardening tissue culture seedling,age of seedling root stock and different concentrations of BA on the graft survival rate were studied. The results showed that the best moisture materials was the parafilm,it was better moisturizing effect and easy to operate;the appropriate training time was 5-6 days;age was two piece of true leaf of seedling root stock and concentrations of BA was 15 mg/L,the graft survival rate was the highest with 91.4%.