大黄酸对2型糖尿病模型大鼠的保护作用

Effects of Rhein on Urinary Protein Excretion in Type 2 Diabetic Model Rats

目的:研究大黄酸对2型糖尿病模型大鼠的保护作用。方法:腹腔注射链脲佐菌素联合高脂饲养以复制大鼠2型糖尿病模型。实验分为正常对照(等容生理盐水)组、模型(等容生理盐水)组、吡格列酮(10 mg/kg)组、大黄酸(100 mg/kg)组。灌胃给药,每天1次,连续8周。测定大鼠尿量(UV)、体质量(BW)、肾质量(KW)、甘油三酯(TG)、总胆固醇(TC)、空腹血糖(FPG)、空腹胰岛素(Fins)、肌酐(Cr)含量,尿蛋白(UPRO)、尿白蛋白(UALB)量,计算肾脏肥大指数(KW/BW)、胰岛素抵抗指数(HOMA-IR)、尿蛋白/肌酐比值(PCR)、尿白蛋白/肌酐比值(ACR)、内生肌酐清除率(Ccr)。结果:与正常对照组比较,模型组大鼠UV、BW、KW、TG、TC、FPG、FINS、Cr、UPRO、UALB量增加,KW/BW、HOMA-IR、PCR、ACR、Ccr升高,差异有统计学意义(P<0.01);与模型组比较,大黄酸组大鼠KW、TG、TC、FPG、Fins、UPRO、UALB、Cr量减少,KW/BW、Ccr升高,HOMA-IR、PCR、ACR降低,差异有统计学意义(P<0.01或P<0.05)。结论:大黄酸具有降低2型糖尿病模型大鼠尿蛋白排泄的作用,其机制可能与改善胰岛素抵抗有关。

OBJECTIVE： To study the effects of rhein on urinary protein excretion in diabetic rats. METHODS： The diabetic model was induced by intraperitoneal injection of streptozotocin （STZ） and high-fat diet. The test were randomly divided into normal control group （constant volume of normal saline）, model group （constant volume of normal saline）, pioglitazone group （10 mg/kg） and rhein group （100 mg/kg）. They were given relevant medicine intragastrically once a day for consecutive 8 weeks. The urinary production （UV）, body weight （BW）, kidney weight （KW）, blood triglycerides （TG）, blood cholesterol （TC）, fasting glucose （FPG）, fasting insulin（Fins）, serum creatinine（Cr）, urine protein（UPRO）and urine albumin（UALB）were all determined, and renal hypertrophy index （KW/BW）, insulin resistance index （HOMA-IR）, creatinine clearance rate （Ccr）, urine protein-to-creatinine ratio （PCR） and urinary albumin-to-creatinine ratio （ACR） were calculated. RESULTS： Compared with normal control group, above all index of model group increased; there was statistical significance （P〈0.01）. Compared with model group, the level of KW/BW and Ccr in rhein group increased while other index decreased; there was statistical significance （P〈0.01 or P〈0.05）. CONCLUSIONS： Rhein can reduce urinary protein excretion in diabetic rats; its mechanism may be related to the improvement of insulin resistance.