黄芩素体外抑菌与体内抗炎作用研究

Antibacterial Activity in vitro and Anti-inflammatory Effects in vivo of Baicalein

目的:研究黄芩素体外抑菌与体内抗炎作用。方法:采用滤纸片研究黄芩素(10 mg/ml)对大肠埃希菌、金黄色葡萄球菌、铜绿假单胞菌、白色念珠菌、黑曲霉的抑制作用;采用试管二倍稀释法测定黄芩素对上述5种菌株的最低抑菌浓度(MIC)。二甲苯诱发小鼠急性炎症,测定小鼠耳廓肿胀度与小鼠血清肿瘤坏死因子(TNF)-α、白细胞介素(IL)-1含量。结果:10 mg/ml黄芩素对5种菌株具有一定的抑制作用。5种菌株MIC分别为0.312 5、0.312 5、0.625 0、0.312 5、0.625 0 mg/ml。0.20、0.10 mg/kg黄芩素可明显抑制模型小鼠耳廓肿胀,0.20、0.10 mg/kg黄芩素可明显降低模型小鼠血清TNF-α含量,0.20 mg/kg黄芩素可明显降低模型小鼠血清IL-1含量。结论:黄芩素具有一定的体外抗菌与体内抗炎作用。

OBJECTIVE： To study the antibacterical activity in vitro and anti-inflammatory effects in vivo of baicalein. METHODS：Filter paper was used to study inhibitory effects of baicalein （10 mg/ml） on Esckerichia coli, Stapkylococcus aureus, Pseudomonas aeruginosa, Candida albicans and ,Aspergillus niger. MIC of baicalein to 5 strains was determined by tube double dilution method. Xylene induced mice acute inflammation, and mice ear swelling, swelling inhibition rate and serum contents of TNF-α and IL-1 were all determined. RESULTS： 10 mg/ml Baicalein had inhibitory effects on 5 strains. MIC of baicalein to the 5 Strains were 0.312 5, 0.312 5, 0.625 0, 0.312 5 and 0.625 0 mg/ml. 0.2, 0.1 mg/kg baicalein inhibited xylene induced mice ear swelling significantly, and 0.2, 0.1 mg/kg baicalein can significantly reduce the serum content of TNF-α in xylene induced mice; 0.2 mg/kg baicalein can significantly decrease the serum content of IL-1 in xylene induced mice： CONCLUSIONS： Baicalein has antibacterial activity in vitro and anti-inflammatory effects in vivo.