HPLC法同时测定黑柴胡药材中柴胡皂苷a和d的含量

Simultaneous Determination of Saikosaponin a and d in Bupleurum smithii by HPLC

目的：建立同时测定黑柴胡药材中柴胡皂苷a和d含量的方法。方法：采用高效液相色谱法。色谱柱为Inertsil C18（250mm×4．6mm，5μm），流动相为乙腈-水（38：62，V／V），流速为1．0ml／min，检测波长为210nm。结果：柴胡皂苷a和d的进样量分别在0．42546．375gg和0．13043．250μg范围内与各自峰面积积分值呈良好线性关系（r均为0．9999）；精密度、稳定性和重复性试验的RSD〈2％；平均加样回收率分别为96．66％（RSD=1．11％，n=6）和96．20％（RSD=0．78％，n=6）。结论：该方法简便、重复性好，结果稳定、可靠，可用于黑柴胡药材的质量控制。

OBJECTIVE： To develop a method for the simultaneous determination of saikosaponin a and d in Bupleurum smithii. METHODS： HPLC method was adopted. Inertsil C18（250 mm×4.6 mm, 5μm） column was used with mobile phase consisted of acetonitrile-water （38：62, V/V） at flow rate of 1.0 ml/min. The detection wavelength was set at 210 rim. RESULTS： The linear range of saikosaponin a and d were 0.425-6.375 μg（r=0.999 9） and 0.130-3.250 μg（r=0.999 9）. RSD of precision, stability and reproducibility tests were all lower than 2 %. The average recoveries were 96.66 % （RSD=1.11%, n=6） and 96.20% （RSD = 0.78% ,n=6） for sakosaponin a and d, respectively. CONCLUSIONS： The method is simple, reproducible, stable and reliable. It can be used for the quality control of B. smithii.