电针对非酒精性脂肪肝大鼠血清RBP4水平的影响及其脂质调节作用机制

Influence of electroacupuncture intervention in serum RBP4 level in rats with non-alcoholic fatty liver disease and its lipid regulation mechanism

目的：探讨电针对高脂高胆固醇造模的非酒精性脂肪肝（NAFLD）大鼠的脂质调节作用，阐明血清视黄醇结合蛋白4（RBP4）水平以及肝 X受体α（LXR-α）和肝脏固醇调节元件结合蛋白-1 c （SREBP-1 c）表达的调节作用机制。方法：44只雌性 SD大鼠适应性喂养7 d，随机分为正常组、模型组、东宝肝泰组和电针组，每组11只。正常组大鼠用标准饲料喂养，其余各组大鼠用高脂高胆固醇饲料喂养。8周后电针组针刺“肝俞”、“脾俞”和“膈俞”进行治疗，电压9 V，电流强度1～3 mA，频率为1.5～2.0 Hz，疏密波，留针15 min，每天1次，连续28 d。检测各组大鼠空腹血糖（FBG）、血清游离脂肪酸（FFA）和肝组织匀浆甘油三酯（TC）、总胆固醇（TG）的变化，采用酶联免疫吸附法（ELISA）测定血清 RBP4水平，采用 Western blotting法检测大鼠肝组织中 LXR-α和SREBP-1 c蛋白表达水平。结果：与正常组比较，模型组大鼠的 FBG、FFA、肝组织匀浆中 TC和 TG以及血清 RBP4水平升高（P＜0.01）， LXR-α和 SREBP-1 c蛋白表达水平也升高（P＜0.01）；与模型组比较，电针组和东宝肝泰组大鼠 FBG、FFA、肝组织匀浆TC和TG水平下降（P＜0.05或P＜0.01），血清 RBP4水平下降（P＜0.01）， LXR-α和 SREBP-1 c蛋白表达水平明显降低（P＜0.05或P＜0.01）。结论：电针“肝俞”、“脾俞”、“膈俞”能降低 NAFLD大鼠血清 RBP4水平，调节脂质代谢，改善脂质沉积，对 NAFLD有明显的治疗作用，其机制可能与抑制肝组织中 LXR-α和 SREBP-1 c蛋白表达上调有关。

Objective To observe the lipid regulation role of electroacupuncture intervention in the rats with non-alcoholic fatty liver disease（NAFLD）induced with high fat and cholesteol forage,and to clarify the regulation mechanism of serum retinol binding protein 4 （RBP4 ）level and liver X receptor a （LXR-α）and sterol regulatory element binding protein-1c（SREBP-1c）expressions.Methods 44 female SD rats were fed for 7 d to adapt the environment and were randomly divided into normal group, model group, Dongbaogantai group, electroacupuncture group;11 rats in each group.The rats in normal group got routine feeding,and the others were fed with high fat and high cholesterol forage. After 8 weeks, the models were established, the rats in＆amp;nbsp;electroacupuncture group were treated with electroacupuncture method （1.5-2.0 Hz, D.-D.wave, 9V, 1-3 mA）in“Ganshu”,“Pishu”,“Geshu”for 15 min,once a day,lasted for 28 d.The changes of fasting blood-glucose（FBG）,serum free fatty acids（FFA）and liver tissue homogenate triglyceride（TG）and total cholesterol （TC）levels of the rats in various groups were tested, and enzyme-linked immunosorbent （ELISA）was used to determine the serum RBP4 levels, and Western blotting method was used to detect the LXR-αand SREBP-1 c protein expression levels in rat liver tissue.Results Compared with normal group,the FBG,serum FFA,TG and TC levels in liver tissue homogenate and serum RBP4 level of the rats in model group were increased （P〈0.01）;the LXR-αand SREBP-1c protein expression levels were also increased （P〈0.01）.Compared with model group, the FBG,serum FFA,the TG and TC levels in liver tissue homogenate and serum RBP4 levels of the rats in electroacupuncture group and Dongbaogantai group were decreased （P〈0.01）;the LXR-αand SREBP-1c protein expression levels were also decreased （P〈 0.05 or P〈 0.01 ）. Conclusion Electroacupuncture method in“Fenglong”,“Zusanli”,“Sanyinjiao”can reduce the serum RBP4 level, regulate the lipid metabolism, and improve the lipid deposition of the NAFLD rats;they have obvious therapeutic effect on NAFLD, and its mechanism may be related to inhibiting the increasing of LXR-αand SREBP-1 c protein expressions in liver tissue.