TFPI-2和MMP-9在食管鳞状细胞癌组织中的表达及其与血管生成拟态的关系

Expressions of TFPI-2 and MMP-9 in esophageal squamous cell carcinoma tissue and their relationship with vasculogenic mimicry

目的:探讨组织因子途径抑制剂2(TFPI-2)和基质金属蛋白酶9(MMP-9)在食管鳞状细胞癌中的表达及其与血管生成拟态(VM)的关系。方法:收集手术切除的162例食管鳞状细胞癌标本,采用过碘雪夫酸(PAS)和CD34双重染色法观察VM在食管鳞状细胞癌组织中的分布特征。采用免疫组织化学SP法检测TFPI-2和MMP-9在食管鳞状细胞癌组织中的表达情况。分析VM与食管鳞状细胞癌临床病理参数以及TFPI-2和MMP-9表达的关系。结果:食管鳞状细胞癌组织中VM阳性率为20.37%。低分化组VM阳性率(40.38%)高于中分化组(11.76%)和高分化组(7.14%),3组间比较差异有统计学意义(χ2=20.915,P<0.01)。TNMⅠ-Ⅱ期组VM阳性率(11.59%)低于Ⅲ期组(26.88%)(χ2=5.707,P=0.017)。在低分化组中,TFPI-2在VM(+)组的表达率[33.34%(7/21)]高于VM(-)组[6.45%(2/31)](χ2=4.582,P=0.032);MMP-9在VM(+)组的表达率[78.79%(26/33)]高于VM(-)组[44.96%(58/129)](χ2=12.05,P=0.001);低分化组TFPI-2的表达水平与VM呈正相关关系(r=0.166,P=0.032),MMP-9的表达水平与VM呈正相关关系(r=0.183,P=0.018),VM(-)组5年生存率高于VM(+)组(χ2=22.84,P<0.001)。结论:低分化及晚期食管鳞状细胞癌易形成VM,VM与食管鳞状细胞癌的不良预后有关,且TFPI-2和MMP-9对VM的形成有促进作用。

Objective To investigate the expressions of tissue factor pathway inhibitor-2 （TFPI-2 ） and matrix metalloproteinase-9 （MMP-9）in esophageal squamous cell carcinoma（ESCC）tissue and their relationship with vasculogenic mimicry （VM）.Methods 162 cases of esophageal squamous cell carcinoma tissues were collected. CD34/periodic acid-schiff double staining was performed to observe the distribution of VM in ESCC tissue,and immunohistochemical staining was used to detect the expressions of TFPI-2 and MMP-9 in ESCC tissue. The relationship between VM and the clinicopathologic parameters of ESCC, the expressions of TFPI-2 and MMP-9 were analyzed.Results The positive rate of VM in ESCC tissue was 20.37%.The positive rate of VM in poorly differentiated group（40.38%）was significantly higher than those in moderately differentiated group（11.76%）and well differentiated group （7.14%）（χ2 = 20.915, P 〈 0.01 ）. The positive rate of VM in TNM Ⅰ-Ⅱ（11.59%）group was significantly lower than that in TNM Ⅲ group（26.88%）（χ2=5.707,P=0.017）.The＆amp;nbsp;positive rate of TFPI-2 in VM（＋）group（33.34%）was significantly higher than that in VM（-）group（6.45%） （χ2=4.582,P=0.032）in poorly differentiated ESCC.The positive rate of MMP-9 in VM（＋）group（78.79%） was significantly higher than that in VM（-）group（44.96%）（χ2=12.05,P=0.001）.The expression level of TFPI-2 in poorly differentiated group was positively correlated with VM （r= 0.166,P= 0.032 ）, and the expression level of MMP-9 was positively correlated with the VM（r=0.183,P=0.018）.The five-year survival rate in VM （-） group was significant higher than that in VM （＋） group （χ2 = 22.84, P 〈 0.001 ）. Conclusion VM exists in ESCC tissue,especially in poorly differentiated and advanced ESCC tissue.VM is related to poor prognostis of ESCC.TFPI-2 and MMP-9 might involve in the formation of VM in ESCC.