肝卵圆细胞对大鼠肝硬化组织TGFβ/smad信号传导的影响

The effect of hepatic oval cell transplantation on TGFβ / smad signaling transduction in rats with hepatic cirrhosis

目的探讨肝卵圆细胞对TGFβ/smad信号传导通路影响的机制,并证明肝卵圆细胞对肝硬化的发展是否有阻止和逆转的作用。方法对肝卵圆细胞进行增殖、分离、培养后,移植于肝硬化大鼠肝脏组织,以未经移植的大鼠做对照,分别进行组织病理学和肝功能及蛋白表达水平的检测,分析ALB、AST、ALT、TGFβ1、TGFβRⅡ、Smad2、Smad4、Smad7的情况。结果移植肝卵圆细胞的鼠肝硬化组织纤维化减少,肝功能明显改善(P<0.05),肝硬化组织TGFβ/smad信号通路中各蛋白表达量有差异。结论肝卵圆细胞刺激肝硬化细胞后TGFβ/smad信号通路中各蛋白的表达是不同的,肝卵圆细胞对肝硬化组织有阻止和逆转的作用。

Objective To investigate the role of TGFβ/smad signaling transduction in a rat carbon tetrachloride （CCL4）-induced model of hepatic cirrhosis, and the effect of hepatic oval cells for inhibiting development of hepatic cirrhosis. Methods Hepatic oval cells were cultured, proliferated, separated and transplanted into liver tissue of rats with hepatic cirrhosis. The rats transplanted with hepatic oval cells were assigned as research group, those without hepatic oval cells were served as Control Group. The morphological character by HE staining, and serum ALB, AST and ALT were observed in all animals. Expression of protein TGFβ1, TGFβR Ⅱ , Smad2, Smad4 and Smad7 in liver tissue were measured by western blot. Results The hepatic fibrosis tissue observed in Research Group animals seemed alleviated compared with controls. The levels of serum AST and ALT in research group animals were also lower than that in controls except for serum ALB （P〈0.05）. Meanwhile, Expression of TGFβ1, TGFβR Ⅱ , Smad2, Smad4 in liver tissues of research group were lower than that of controls but Smad7. Conclusion Liver oval cells showed an antifibrotic effect on experimental liver fibrosis.The possible mechanism is that hepatic oval cells inhibit the expression of by TGFβ/smad signaling transdnction.