川产忍冬属三种植物的核型分析

Karyotype Analysis of Three Lonicera Species Growth in Sichuan

目的:为川产忍冬品种的开发应用提供遗传参考。方法:应用改良的染色体制片技术,对忍冬、红白忍冬和细毡毛忍冬的茎尖细胞进行染色体制片和核型分析,并计算相关染色体参数。结果:忍冬、红白忍冬和细毡毛忍冬的染色体数目均为2n=2X=18,染色体总长分别为30.747、33.231和36.948μm;核型公式分别为2n=2X=18=2m+7sm,2n=2X=18=8m+8sm+2st和2n=2X=18=8sm+10m;核不对称系数为64.013%、64.380%和61.949%;核型依次为2B、2B和2A型。结论:3种忍冬遗传进化程度较高,可作为广泛栽培的种质资源。

Objective ：To provide the genetic reference for development and application of Lonicera species growth in Sichuan. Methods ： Shoot apices cells from Lonicera japonica, Lonicera japonica vat. chinensis and Lonicera similis （cultivars）were used to make the chromosomal preparation. Their karyotypes were analyzed and the relevant parameters of chromosomes were measured by improved chromosome preparation technique. Results：The chromosome numbers of three Lonicera species were 2n = 2X = 18;their chromosome length was 30. 747,33. 231 and 36. 948 ixm;Their karyotype formula was 2n =2X = 18 =2m ＋ 7sm,2n =2X = 18 =8m ＋8sm ＋2st and 2n =2X = 18 = 8sm ＋ 10re;Their As. k was 64. 013% ,64. 380% and 61. 949% ;And their karyotypes belonged to 2B,2B and 2A type,respectively. Conclusion ：These three Lonicera species can be of the germplasm resources for widely cultivating since they have high degree of genetic evolution.