壮药排钱草根的抗肝纤维化成分研究

Chemical Constituents Against Hepatic Fibrosis from Phyllodium pulchellum Roots

目的：对壮药排钱草根进行抗肝纤维化活性成分研究。方法：通过多种色谱技术结合光谱学手段对排钱草根中的化学成分进行分离与结构鉴定。利用乙醛诱导的小鼠肝星状细胞（HSC-T6）增殖模型对所得到的化合物进行抗肝纤维化活性评价。结果：从排钱草根中分离鉴定了10个化合物,分别为：3,5,2＇,4＇-tetrahydroxy-2″,2″-dimethylpyrano-[5″,6″,7,8]-flavanone（1）、异柠檬酚（2）、citflavanone（3）、8-prenylated 5,7,3＇,4＇-tetrahydroxy flavanone（4）、pulchelstyrene A（5）、pulchelstyrene B（6）、pulchelstyrene D（7）、3-indolcarbaldehyde（8）、3-methoxyindole（9）、对羟基苯甲酸（10）。结论：其中,化合物1-4、8-10为首次从该植物中分离得到。体外活性评价结果显示化合物2、4-6对乙醛诱导的小鼠肝星状细胞增殖具有一定的抑制作用。

Objective： To investigate the bioactive constituents against hepatic fibrosis from the roots of Phyllodium pulchellum. Methods ： The chemical constituents of Phyllodium pulchellum roots were obtained by various chromatographic technologies and identified by several spectroscopic methods. Results：Ten compounds were elucidated as 3,5,2＇ ,4＇-tetrahydroxy-2″ ,2″-dimethylpyrano- [ 5″, 6 ″, 7,81 -flavanone （ 1 ）, yukovanol （ 2 ）, citflavanone （ 3 ）, 8-prenylated 5,7,3＇, 4 ＇-tetrahydroxyflavanone （ 4 ）, pulchelstyrene A （ 5 ） , pulchelstyrene B （ 6 ） , pulcheIstyrene D （ 7 ） , 3-indolcarbaldehyde （ 8 ） , 3-methoxyindole （ 9 ） and p-hydroxybenzoic acid （ 10 ）. The effects to inhibit the proliferation of activated HSC-T6 cells of all isolated compounds were also evaluated. Conclusion ： All compounds are isolated from this plant for the first time except for compounds 5 - 7. Compounds 2,4,5 and 6 can inhibit the proliferation of activated HSC-T6 cells in vitro.