新型白僵菌热雾剂的生物活性研究

Study on biological activity of thermal aerosol with Beauveria bassiana spores

脉冲烟雾机应用烟雾载药防治技术具有防治效率高、用药量少、环保等诸多优点,但迄今为止只用于化学农药。笔者配制了3种白僵菌制剂,检测其在25℃与50℃高温模拟存储条件下以及热力烟化后的生物活性。结果表明:在50℃高温条件下,存放1 d时的孢子萌发率下降很少,存放3 d时仍高达79.7%以上,存放7 d时最低活性可达53.1%。柴油热雾剂经过脉冲烟雾机热力烟化后的出口烟雾温度远远高于3种白僵菌制剂,且高温处理7 d时的生物活性几乎全部丧失,说明柴油不能直接作为白僵菌热雾剂的溶剂油。3种白僵菌制剂经过脉冲烟雾机高温热力烟化后孢子平均萌发率达到91.3%,只比热力烟化前平均下降了0.87%。说明丙三醇与水按一定比例混合后作为生物农药热雾剂的溶剂是可行的。

The pesticide control technology with pulse fogger has the advantages of high efficiency, less dosage, environ-mental friendly protection and others. However, it is only applied with chemical pesticides. In the paper, germination a-bility of three Beauveria bassiana preparations were tested, stored separately in temperature-controlled chamber with 25 ℃ and 50℃, and after thermal smoking. Test results on the biological activity of B. bassiana spores shown that spore germination ability decreased slightly after a day of storage, and was more than 79.7% after 3 days of storage, and reached 53.1% after 2 days of storage, in the condition of 50 ℃ high temperature simulation test. The fog temperature for diesel located on the fog ejector of pulse thermal fogger was greatly higher than the three B. bassiana preparations. So, it showed that the diesel could not be used directly as solvent oil of thermal aerosol with B. bassiana. After high tem-perature thermal atomization, the biological activity of B. bassiana preparations didn.t decrease obviously, which the fall value was only 0.87%. It was shown that mixed solution of glycerol and water in suitable proportion was feasible to apply in the solvent of thermal aerosol with bio-pesticide.