基于trnL-F基因序列的铁兰属植物亲缘关系分析

Phylogenetic relationship analysis of Tillandsia plants based on trnL-F gene

以莺哥凤梨（Vriesea carinata）为外类群，利用Clustal X1．81和MEGA3．1软件对31种铁兰属植物的基因序列进行比对和分析，并构建分子系统树。结果表明：31种铁兰属植物供试材料的trnL-F序列长度在806～835 bp之间，变异位点22个，信息位点14个，平均遗传距离0．007，且在301～322 bp和826～830 bp处分别出现了插入和polyT序列。利用ML法建立的系统树显示，31个铁兰属植物分为3组。序列分析结果与形态学结果基本一致，表明trnL-F序列可用于铁兰属植物的亲缘关系分析，并可作为某些特定种的鉴定依据。

In this paper, Catopsis subulata was selected as the outgroup, and trnL-F genes of 31 Tillandsia species were analyzed and compared with each other to construct the phylogenetic trees by use of Clusta1 X1？81 and MEGA3.1 soft-wares. The results showed that the length of trnL-F genes in 31 Tillandsia plants were in the range of 806 bp to 835 bp, and there were 22 variable sites and 14 informative sites among them. On the other hand, the average genetic distance of 31 trnL-F gene sequences was 0.007, and at the points of 301-322 bp,there were 21 deoxyribonucleotides inserted in trnL-F gene sequences from T.fasciculata and T.tenuifolia,and a polyT sequence appeared at 826-830 bp.The phyloge-netic trees built by ML method illustrated that 31 species could be clustered together into three groups. The clustered re-sults were consistent with the morphologic identification. This indicated that trnL-F gene could be used for the Tillandsia relationship analysis and as the basis for the identification of the specific species.